Paired Whole Cell Recordings in Organotypic Hippocampal Slices

Chantelle Fourie; Marianna Kiraly; Daniel V. Madison; Johanna M. Montgomery

doi:10.3791/51958

ジャーナル

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神経科学

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器官型海馬スライスにおけるペア全細胞記録

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

JoVE Journal
神経科学

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JoVE Journal 神経科学

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

器官型海馬スライスにおけるペア全細胞記録

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14,645 Views

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09:23 min

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September 28, 2014

DOI:

10.3791/51958-v

DOI:

10.3791/51958-v

•

09:23 min

September 28, 2014

•

5 Views

Chantelle Fourie¹, Marianna Kiraly², Daniel V. Madison*², Johanna M. Montgomery*¹

¹Department of Physiology and Centre for Brain Research,University of Auckland, ²Department of Molecular and Cellular Physiology,Stanford University

概要

Pair recordings are simultaneous whole cell patch clamp recordings from two synaptically connected neurons, enabling precise electrophysiological and pharmacological characterization of the synapses between individual neurons. Here we describe the detailed methodology and requirements for establishing this technique in organotypic hippocampal slice cultures in any laboratory equipped for electrophysiology.