An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, <em>Aedes albopictus</em>

Monica F. Poelchau; Xin Huang; Allison Goff; Julie Reynolds; Peter Armbruster

doi:10.3791/51961

ジャーナル

/

生物学

/

アジアのタイガーモスキートにおける日長休眠のRNA-seqの分析のための実験とバイオインフォマティクス·プロトコル、<em>ヒトスジシマカ</em

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, <em>Aedes albopictus</em>

JoVE Journal
生物学

このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。

JoVE Journal 生物学

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

アジアのタイガーモスキートにおける日長休眠のRNA-seqの分析のための実験とバイオインフォマティクス·プロトコル、<em>ヒトスジシマカ</em

Please note that all translations are automatically generated. Click here for the English version.

13,322 Views

•

12:10 min

•

November 30, 2014

DOI:

10.3791/51961-v

DOI:

10.3791/51961-v

•

12:10 min

November 30, 2014

•

13266 Views

Monica F. Poelchau¹, Xin Huang¹, Allison Goff¹, Julie Reynolds², Peter Armbruster¹

¹生物学科,Georgetown University ²Insect Physiology Lab, EEOB,The Ohio State University

概要

RNA-Seq analyses are becoming increasingly important for identifying the molecular underpinnings of adaptive traits in non-model organisms. Here, a protocol to identify differentially expressed genes between diapause and non-diapause Aedes albopictus mosquitoes is described, from mosquito rearing, to RNA sequencing and bioinformatics analyses of RNA-Seq data.