From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Carmine Di Rienzo; Enrico Gratton; Fabio Beltram; Francesco Cardarelli

doi:10.3791/51994

ジャーナル

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生物工学

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商業顕微鏡でのライブ細胞膜上の分子拡散法への高速蛍光イメージングから

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

JoVE Journal
生物工学

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JoVE Journal 生物工学

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

商業顕微鏡でのライブ細胞膜上の分子拡散法への高速蛍光イメージングから

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11,409 Views

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15:10 min

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October 09, 2014

DOI:

10.3791/51994-v

DOI:

10.3791/51994-v

•

15:10 min

October 09, 2014

•

11389 Views

Carmine Di Rienzo^1,2, Enrico Gratton³, Fabio Beltram¹, Francesco Cardarelli²

¹NEST Laboratory,Scuola Normale Superiore ²Center for Nanotechnology Innovation,Instituto Italiano di Tecnologia ³Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering,University of California, Irvine

概要

Spatial distribution and temporal dynamics of plasma membrane proteins and lipids is a hot topic in biology. Here this issue is addressed by a spatio-temporal image fluctuation analysis that provides conceptually the same physical quantities of single particle tracking, but it uses small molecular labels and standard microscopy setups.