Murine Isolated Heart Model of Myocardial Stunning Associated with Cardioplegic Arrest

Brenda Cordeiro; Richard Clements

doi:10.3791/52433

JoVE Journal
Medicine

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JoVE Journal Medicine

Murine Isolated Heart Model of Myocardial Stunning Associated with Cardioplegic Arrest

心臓麻痺逮捕と気絶心筋関連のマウス単離された心臓モデル

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08:30 min

August 6, 2015

DOI: 10.3791/52433-v

Brenda Cordeiro¹, Richard Clements^1,2

¹Cardiothoracic Surgery Research Center,Brown University, ²Rhode Island Hospital and Alpert Medical School,Brown University

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Overview

This protocol assesses myocardial stunning following ischemic cardioplegic arrest in rodents. It involves retrograde perfusion of a harvested heart and monitoring myocardial function recovery after ischemic injury.

Key Study Components

Area of Science

Cardiovascular physiology
Ischemic injury
Rodent models

Background

Myocardial stunning is a temporary loss of function following ischemia.
Understanding this phenomenon can aid in developing therapeutic strategies.
Rodent models are commonly used for cardiovascular research.
This method can also be applied to other mammals.

Purpose of Study

To evaluate the effects of ischemic cardioplegic arrest on myocardial function.
To monitor recovery post-ischemia.
To provide insights into myocardial stunning mechanisms.

Methods Used

Harvesting a heart from an adult mouse.
Retrograde perfusion of the heart.
Insertion of a balloon into the left ventricle.
Recording baseline control measurements and initiating hypothermic cardioplegic arrest.

Main Results

Monitoring changes in myocardial function during recovery.
Assessing the impact of ischemic injury on heart performance.
Demonstrating the applicability of the method to other mammalian models.
Providing insights into mild ischemic insults.

Conclusions

This technique effectively assesses myocardial stunning.
It can enhance understanding of ischemic heart conditions.
Potential for broader application in cardiovascular research.

Frequently Asked Questions

What is myocardial stunning?

Myocardial stunning is a temporary loss of heart function following ischemia.

How is the heart prepared for the experiment?

The heart is harvested from an adult mouse and retrograde perfused.

What measurements are taken during the study?

Baseline control measurements are recorded before initiating cardioplegic arrest.

Can this method be applied to other animals?

Yes, it can also be applied to rats and other mammals.

What is the significance of this research?

It provides insights into myocardial function recovery after ischemic injury.

What are the implications for therapeutic strategies?

Understanding myocardial stunning can aid in developing treatments for ischemic heart conditions.

このプロトコルの目標は、げっ歯類の虚血性心筋麻痺後の心筋の気絶を評価することです。

この手順の全体的な目標は、げっ歯類の虚血性心筋麻痺停止後に発生する心筋の気絶を評価することです。これは、成体マウスから採取した心臓である最初の逆行性灌流によって達成されます。2 番目のステップでは、バルーンを心臓の左心室に挿入し、ベースライン制御測定値を記録します。

その後、低体温性心筋麻痺の停止が適切な実験期間に開始され、その後、再灌流期間が続きます。最終的に、この手法は、虚血性損傷後の心筋機能の回復中に発生する変化を監視するために使用できます。この方法は、マウスの軽度の虚血性傷害に関連する心筋の気絶についての洞察を提供することができますが、ラットや重度の虚血再灌流障害の2つのモデルなどの他の哺乳類にも適用できます。

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