Immunostaining to Visualize Murine Enteric Nervous System Development

Amanda J. Barlow-Anacker; Christopher S. Erickson; Miles L. Epstein; Ankush Gosain

doi:10.3791/52716

ジャーナル

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発生生物学

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マウス腸神経系の開発を可視化するために免疫染色

JoVE Journal
発生生物学

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JoVE Journal 発生生物学

Immunostaining to Visualize Murine Enteric Nervous System Development

マウス腸神経系の開発を可視化するために免疫染色

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11,292 Views

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07:54 min

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April 29, 2015

DOI:

10.3791/52716-v

DOI:

10.3791/52716-v

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07:54 min

April 29, 2015

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5 Views

Amanda J. Barlow-Anacker¹, Christopher S. Erickson¹, Miles L. Epstein², Ankush Gosain^1,2,3

¹外科,University of Wisconsin School of Medicine and Public Health, ²Department of Neurosciences,University of Wisconsin School of Medicine and Public Health, ³小児科,University of Wisconsin School of Medicine and Public Health

概要

The enteric nervous system is formed by neural crest cells that proliferate, migrate and colonize the gut. Neural crest cells differentiate into neurons with markers specific for their neurotransmitter phenotype. This protocol describes a technique for dissecting, fixing and immunostaining of the murine embryonic gastrointestinal tract to visualize enteric nervous system neurotransmitter expression.