Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

Laura M. Ketelboeter; Sonia L. Bardy

doi:10.3791/53105

ジャーナル

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免疫学と感染

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細菌Pyomelanin産生を阻害し、酸化ストレスに対する感受性の増加に対応を決定するための方法

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

JoVE Journal
免疫学と感染

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JoVE Journal 免疫学と感染

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

細菌Pyomelanin産生を阻害し、酸化ストレスに対する感受性の増加に対応を決定するための方法

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8,134 Views

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11:00 min

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August 31, 2015

DOI:

10.3791/53105-v

DOI:

10.3791/53105-v

•

11:00 min

August 31, 2015

•

2 Views

Laura M. Ketelboeter¹, Sonia L. Bardy¹

¹Department of Biological Sciences,University of Wisconsin Milwaukee

概要

Bacterial pyomelanin production results in increased resistance to oxidative stress and virulence. We report on techniques that can be used to determine inhibition of pyomelanin production and assay the resulting increase in sensitivity to oxidative stress in bacteria, as well as determine antibiotic minimum inhibitory concentration (MIC).