Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

Sarah J. Potter; Tony DeFalco

doi:10.3791/53262

ジャーナル

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発生生物学

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使い方エクスビボ全体の胎児器官の直立飛沫培養物をマウス器官中に発生過程を研究します

Using <em>Ex Vivo</em> Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

JoVE Journal
発生生物学

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JoVE Journal 発生生物学

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

使い方エクスビボ全体の胎児器官の直立飛沫培養物をマウス器官中に発生過程を研究します

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12,417 Views

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09:47 min

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October 21, 2015

DOI:

10.3791/53262-v

DOI:

10.3791/53262-v

•

09:47 min

October 21, 2015

•

1 Views

Sarah J. Potter¹, Tony DeFalco¹

¹Division of Reproductive Sciences,Cincinnati Children’s Hospital Medical Center

概要

The ex vivo upright droplet culture is an alternative to current in vitro and in vivo experimental techniques. This protocol is easy to perform and requires smaller amounts of reagent, while permitting the ability to manipulate and study fetal vascularization, morphogenesis, and organogenesis.