Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Rabeea H. Mohammed; Dylan Sweetman

doi:10.3791/53342

ジャーナル

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発生生物学

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遺伝子発現に影響を及ぼすシグナル伝達経路を同定するために、ニワトリ胚手足の開発にビーズのグラフト

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

JoVE Journal
発生生物学

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JoVE Journal 発生生物学

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

遺伝子発現に影響を及ぼすシグナル伝達経路を同定するために、ニワトリ胚手足の開発にビーズのグラフト

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8,808 Views

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11:48 min

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January 17, 2016

DOI:

10.3791/53342-v

DOI:

10.3791/53342-v

•

11:48 min

January 17, 2016

•

8791 Views

Rabeea H. Mohammed¹, Dylan Sweetman¹

¹Division of Animal Sciences, School of Biosciences,University of Nottingham

概要

By grafting beads soaked in growth factors or specific inhibitors of signaling pathways into developing embryos it is possible to directly test their effects in vivo. In this protocol beads are grafted into the limb bud to determine the effects of these molecules on gene expression and signal transduction.