A Behavioral Assay for Mechanosensation of MARCM-based Clones in <em>Drosophila melanogaster</em>

Timothy P. Murphy; Dan D. Luu; Jessica A. DeSimone; Thomas C. O'Brien; Christopher J. Lally; Jillian J. Lindblad; Sarah M. Webster

doi:10.3791/53537

ジャーナル

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神経科学

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でMARCMベースのクローンのMechanosensationための行動アッセイ<em>キイロショウジョウバエ</em

A Behavioral Assay for Mechanosensation of MARCM-based Clones in <em>Drosophila melanogaster</em>

JoVE Journal
神経科学

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JoVE Journal 神経科学

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

でMARCMベースのクローンのMechanosensationための行動アッセイ<em>キイロショウジョウバエ</em

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10,175 Views

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05:48 min

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December 30, 2015

DOI:

10.3791/53537-v

DOI:

10.3791/53537-v

•

05:48 min

December 30, 2015

•

2 Views

Timothy P. Murphy^1,2, Dan D. Luu¹, Jessica A. DeSimone^1,3, Thomas C. O’Brien^1,4, Christopher J. Lally^1,5, Jillian J. Lindblad^1,6, Sarah M. Webster¹

¹生物学科,College of the Holy Cross, ²School of Medicine,Georgetown University, ³Department of Biochemistry,Giesel School of Medicine, Dartmouth College, ⁴School of Medicine,Tufts University, ⁵Transgenomic Inc., ⁶Department of Molecular, Cell and Cancer Biology,UMass Medical School

概要

In order to identify novel mutations affecting mechanosensation, we designed an assay that measures the behavioral response to tactile stimulation of fly bristles in mutant clones generated by the MARCM method. The combination of techniques allows for the identification of mechanosensitive mutations that would otherwise be lethal.