Isolation and Characterization of Single Cells from Zebrafish Embryos

Leigh Ann Samsa; Nicole Fleming; Scott Magness; Li Qian; Jiandong Liu

doi:10.3791/53877

ジャーナル

/

発生生物学

/

ゼブラフィッシュ胚からの単一細胞の単離とキャラクタリゼーション

Isolation and Characterization of Single Cells from Zebrafish Embryos

JoVE Journal
発生生物学

このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。

JoVE Journal 発生生物学

Isolation and Characterization of Single Cells from Zebrafish Embryos

ゼブラフィッシュ胚からの単一細胞の単離とキャラクタリゼーション

Please note that all translations are automatically generated. Click here for the English version.

14,518 Views

•

09:25 min

•

March 12, 2016

DOI:

10.3791/53877-v

DOI:

10.3791/53877-v

•

09:25 min

March 12, 2016

•

14494 Views

Leigh Ann Samsa^1,2, Nicole Fleming^2,3, Scott Magness¹, Li Qian^2,3, Jiandong Liu^2,3

¹Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill ²McAllister Heart Institute,University of North Carolina at Chapel Hill ³Department of Pathology and Laboratory Medicine,University of North Carolina at Chapel Hill

概要

This protocol describes a method for isolating single cells from zebrafish embryos, enriching for cells of interest, capturing zebrafish cells in microfluidic based single cell multiplex systems, and assessing gene expression from single cells.

Explore More Videos

Single Cell Isolation Zebrafish Embryos Microfluidic-based Single-cell Analysis Gene Expression 発生生物学 Cell Heterogeneity Cell Dissociation Cell Preparation Chorion Removal De-yolking Cell Washing Cell Resuspension

ゼブラフィッシュ胚からの単一細胞の単離とキャラクタリゼーション

概要

Automatically generated

Explore More Videos

関連ビデオ