High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

Alexander Braun; Nicole M. Caesar; Kyvan Dang; Kenneth A. Myers

doi:10.3791/54265

ジャーナル

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生物学

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リビングヒト臍帯静脈内皮細胞における高解像度タイムラプスイメージングと微小管ダイナミクスの自動解析

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

JoVE Journal
生物学

This content is Free Access.

JoVE Journal 生物学

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

リビングヒト臍帯静脈内皮細胞における高解像度タイムラプスイメージングと微小管ダイナミクスの自動解析

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11,016 Views

•

10:25 min

•

August 13, 2016

DOI:

10.3791/54265-v

DOI:

10.3791/54265-v

•

10:25 min

August 13, 2016

•

9 Views

Alexander Braun¹, Nicole M. Caesar¹, Kyvan Dang¹, Kenneth A. Myers¹

¹Department of Biological Sciences,University of the Sciences in Philadelphia

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概要

Protocols for Human Umbilical Vein Endothelial Cell (HUVEC) culture, transient transfection of fluorescently-labeled markers of microtubule growth, live-cell imaging and automated analysis of interphase microtubule growth dynamics are detailed.

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リビングヒト臍帯静脈内皮細胞における高解像度タイムラプスイメージングと微小管ダイナミクスの自動解析

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概要

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