Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Nicolaas H. Fourie; Ralph M. Peace; Sarah K. Abey; LeeAnne B. Sherwin; John W. Wiley; Wendy A. Henderson

doi:10.3791/54693

ジャーナル

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遺伝学

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過敏性腸症候群におけるmiRNA循環の摂動は多重化ハイスループット遺伝子発現プラットフォームを使用して検出します

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

JoVE Journal
遺伝学

This content is Free Access.

JoVE Journal 遺伝学

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

過敏性腸症候群におけるmiRNA循環の摂動は多重化ハイスループット遺伝子発現プラットフォームを使用して検出します

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8,215 Views

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10:37 min

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November 30, 2016

DOI:

10.3791/54693-v

DOI:

10.3791/54693-v

•

10:37 min

November 30, 2016

•

9 Views

Nicolaas H. Fourie¹, Ralph M. Peace^1,2, Sarah K. Abey¹, LeeAnne B. Sherwin¹, John W. Wiley³, Wendy A. Henderson¹

¹Digestive Disorders Unit, National Institute of Nursing Research,National Institutes of Health, DHHS, ²National Institutes of Health Research Scholar,Howard Hughes Medical Institute, ³Internal Medicine, Medical School,University of Michigan

筆記録

概要

We describe the use of a multiplexed high-throughput gene expression platform that quantitates gene expression by barcoding and counting molecules in biological substrates without the need for amplification. We used the platform to quantitate microRNA (miRNA) expression in whole blood in subjects with and without irritable bowel syndrome.