Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Masaki Miya; Toshifumi Minamoto; Hiroki Yamanaka; Shin-ichiro Oka; Keiichi Sato; Satoshi Yamamoto; Tetsuya Sado; Hideyuki Doi

doi:10.3791/54741

ジャーナル

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環境学

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ろ過水のサンプルおよび環境DNAの抽出のためのフィルターカートリッジの使用

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

JoVE Journal
環境学

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JoVE Journal 環境学

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

ろ過水のサンプルおよび環境DNAの抽出のためのフィルターカートリッジの使用

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24,903 Views

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08:04 min

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November 25, 2016

DOI:

10.3791/54741-v

DOI:

10.3791/54741-v

•

08:04 min

November 25, 2016

•

28 Views

Masaki Miya¹, Toshifumi Minamoto², Hiroki Yamanaka³, Shin-ichiro Oka⁴, Keiichi Sato⁴, Satoshi Yamamoto², Tetsuya Sado¹, Hideyuki Doi⁵

¹Department of Ecology and Environmental Sciences,Natural History Museum and Institute, Chiba, ²Graduate School of Human Development and Environment,Kobe University, ³Faculty of Science and Technology,Ryukoku University, ⁴Okinawa Churashima Research Center, ⁵Graduate School of Simulation Studies,University of Hyogo

筆記録

概要

We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.