Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

Jonathan Fischer; Roger A. Johnson; Elizabeth Boon

doi:10.3791/55129

ジャーナル

/

生化学

/

ニトロセルロース結合アッセイを用いて細菌ヒスチジンキナーゼ自己リン酸化の定量化

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

JoVE Journal
生化学

このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。

JoVE Journal 生化学

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

ニトロセルロース結合アッセイを用いて細菌ヒスチジンキナーゼ自己リン酸化の定量化

Please note that all translations are automatically generated. Click here for the English version.

8,490 Views

•

09:17 min

•

January 11, 2017

DOI:

10.3791/55129-v

DOI:

10.3791/55129-v

•

09:17 min

January 11, 2017

•

1 Views

Jonathan Fischer¹, Roger A. Johnson², Elizabeth Boon¹

¹化学科,Stony Brook University, ²Department of Physiology and Biophysics,Stony Brook University

筆記録

概要

We report and demonstrate an optimized nitrocellulose binding assay that can be used to quantify autophosphorylation of purified bacterial histidine kinases. Our method has several advantages over traditional SDS-PAGE based techniques, providing a valuable alternative for characterizing these important proteins.