窒素キャビテーションと差動遠心分離培養細胞の周辺膜蛋白質の分布を監視することができます。
16,104 Views
•
08:24 min
•
August 18, 2017
DOI:
概要
Automatically generated
超遠心法による窒素のキャビテーションとゾル性細胞質と膜結合蛋白質のそれに続く分離に基づく哺乳類細胞の洗剤無料均質化のためのプロトコルをご紹介します。この方法は可溶性の周辺膜蛋白質の分割監視に最適と膜の一部分。
関連ビデオ
06:53
Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation
関連ビデオ
13592 Views
07:31
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
関連ビデオ
5934 Views
10:19
Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification
関連ビデオ
3875 Views
11:14
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
関連ビデオ
11708 Views
06:26
Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography
関連ビデオ
2917 Views
11:44
Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
関連ビデオ
14083 Views
09:54
Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
関連ビデオ
10788 Views
10:02
Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
関連ビデオ
3986 Views
01:30
Bacterial Culture Filtrate Protein Concentration using an Ultrafiltration Device
関連ビデオ
202 Views
01:05
Centrifugation
関連ビデオ
2144 Views
Read Article
Cite this Article
Zhou, M., Philips, M. R. Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells. J. Vis. Exp. (126), e56037, doi:10.3791/56037 (2017).
Copy