ひと神経前駆細胞 (Npc) における神経発達学的表現型の迅速な検出
9.5K 回視聴
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15 件の引用
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10:47 分
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2018年3月2日
DOI :10.3791/56628-v
2018年3月2日
•9.5K 回視聴
Madeline Williams*1 , Smrithi Prem*1 , Xiaofeng Zhou1 , Paul Matteson2 , Percy Luk Yeung3 , Chi-Wei Lu3 , Zhiping Pang4 , Linda Brzustowicz5 , James H. Millonig2 , Emanuel Dicicco-Bloom1
1Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, 2Center for Advanced Biotechnology and Medicine, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, 3The Child Health Institute of NJ, Department of Obstetrics, Gynecology, and Reproductive Services, Rutgers Robert Wood Johnson Medical School, 4The Child Health Institute of NJ, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, 5Department of Genetics, Rutgers University
* These authors contributed equally
増殖、移行、および神経突起伸長はしばしば脳神経疾患で摂動など発達のプロセスします。このように、迅速かつ再現性をもって人間の iPSC 由来の Npc でこれらの神経発達のプロセスを評価するためのプロトコルを提案する.これらのプロトコルには、NPC 発育に及ぼす関連成長因子と治療の評価ができます。
Chapters in this video
0:04
Title
1:08
Collecting NPCs for Plating of Experimental Conditions
2:30
Neural Precursor Cell DNA Synthesis Assay
3:47
S Phase Entry Assay
4:39
Neural Precursor Cell Number Assay
5:41
Neural Precursor Cell Neurosphere Migration Assay
7:58
Results: Use of Phase-contrast and Fluorescence Microscopy to Study the Proliferation, Migration, and Neurite Outgrowth of Neural Precursor Cells
9:51
Conclusion
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