蛍光寿命イメージングによる細胞内のスネアを可視化
9,583 Views
•
08:55 min
•
December 29, 2017
DOI:
概要
Automatically generated
このプロトコルでは、蛍光共鳴エネルギー移動し、蛍光寿命イメージングに基づく SNARE タンパク質の複合体形成の定量的可視化を可能にする新しいメソッドについて説明します。
関連ビデオ
15:10
From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
関連ビデオ
11427 Views
11:26
Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
関連ビデオ
9303 Views
13:47
TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
関連ビデオ
10805 Views
10:58
SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
関連ビデオ
10861 Views
08:15
Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
関連ビデオ
8081 Views
09:59
Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
関連ビデオ
8829 Views
08:17
Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
関連ビデオ
1859 Views
N/A
Live Fluorescence, Inverse Imaging of Cell Ruffling, and Macropinocytosis
関連ビデオ
2028 Views
05:47
Optimizing Visualization of Axonal Transport of Endogenous Cargo by Fluorescence Microscopy in Living Caenorhabditis elegans
関連ビデオ
761 Views
08:55
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
関連ビデオ
9.6K Views
Read Article
Cite this Article
Verboogen, D. R. J., Baranov, M. V., ter Beest, M., van den Bogaart, G. Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy. J. Vis. Exp. (130), e56745, doi:10.3791/56745 (2017).
Copy