細胞毒性の評価と肺癌精密カット スライスで物質の免疫調節効果
17.5K 閲覧数
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被引用数 60
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12:04 分
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2018年5月9日
DOI :10.3791/57042-v
2018年5月9日
•17.5K 閲覧数
Vanessa Neuhaus*1 , Olga Danov*1 , Sebastian Konzok1 , Helena Obernolte1 , Susann Dehmel1 , Peter Braubach2 , Danny Jonigk2 , Hans-Gerd Fieguth3 , Patrick Zardo4 , Gregor Warnecke4 , Christian Martin5 , Armin Braun1,6 , Katherina Sewald1
1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology, RWTH Aachen University, 6Institute for Immunology, Hannover Medical School
* These authors contributed equally
3 r 原則の観点から動物実験に代わる方法として呼吸モデルが進化しています。呼吸器系物質のリスク評価の特に適切な試金の不足があります。ここでは、浮遊物質の評価のための肺癌精密カット スライスの使用について述べる。
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Chapters in this video
0:05
Title
1:26
General Preparation of Human PCLS and Subsequent Exposure to Chemicals
4:28
Measurement of Chemically-induced Cytotoxicity in Human PCLS by WST-1 Assay
6:01
LDH Assay
7:29
Microscopic Viability Assay with Confocal Laser Scanning Microscope (cLSM)
9:49
Results: Representative Images of the Three-dimensional Microscopic Staining and Image Rendering of Human PCLS
10:59
Conclusion
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