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09:19 min
March 26, 2018
DOI:
10.3791/57315-v
ゲル seq の両方 DNA と RNA-seq 単純なハイドロゲル デバイスを用いた 100-1000 細胞からごくわずかな追加コストでライブラリを同時に準備することも可能。 にします。対ライブラリを生成する生物のプロトコルと同様に、デバイス作製のための詳細なアプローチを提案する.
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Hoople, G. D., Richards, A., Wu, Y., Pisano, A. P., Zhang, K. Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices. J. Vis. Exp. (133), e57315, doi:10.3791/57315 (2018).
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