ショウジョウバエのティッシュの最小限の量を使用してのグリセリンの繊維でミトコンドリアの酸素消費量の測定
11,965 Views
•
14:55 min
•
April 07, 2018
DOI:
筆記録
Automatically generated
The overall goal of this procedure is to evaluate mitochondrial respiration in the common fruit fly, Drosophila Melanogaster, using high resolution respirometry.This is achieved by first dissecting the Drosophila, to collect the thorax.The thoraces containing the flight muscles are then carefully permeabilized mechanically, with sharp forceps, and incubated with saponin, for further permeabilization.The thoraces are then transferred into the chambers of a respirometer, and mitochondrial oxygen consumption is measured with a combination of different substrates, uncouplers and inhibitors.Hi, I am Chlo
概要
Automatically generated
本稿で、ショウジョウバエのグリセリン thoraxes による高解像度の呼吸の酸素消費量を測定する方法を説明します。この手法は古典的なミトコンドリアの分離法と比較して組織の最小限の量を必要として得られた結果、もっと生理学的に関連します。
関連ビデオ
10:39
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
関連ビデオ
13773 Views
N/A
An Affordable and Efficient "Homemade" Platform for Drosophila Behavioral Studies, and an Accompanying Protocol for Larval Mitochondrial Respirometry
関連ビデオ
926 Views
08:19
Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry
関連ビデオ
2480 Views
09:20
Analyzing Mitochondrial Function in a Drosophila melanogaster PINK1B9-Null Mutant Using High-resolution Respirometry
関連ビデオ
983 Views
03:57
Assessment of Mitochondrial Oxygen Consumption Using a Plate Reader-based Fluorescent Assay
関連ビデオ
617 Views
14:55
Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue
関連ビデオ
12.0K Views
08:10
Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans
関連ビデオ
9.8K Views
03:57
Assessment of Mitochondrial Oxygen Consumption Using a Plate Reader-based Fluorescent Assay
関連ビデオ
617 Views
11:30
Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers
関連ビデオ
25.5K Views
07:41
Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice
関連ビデオ
4.3K Views
Read Article
Cite this Article
Simard, C. J., Pelletier, G., Boudreau, L. H., Hebert-Chatelain, E., Pichaud, N. Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue. J. Vis. Exp. (134), e57376, doi:10.3791/57376 (2018).
Copy