25,262 Views
•
09:36 min
April 10, 2018
DOI:
10.3791/57379-v
この RNA プルダウン法は、長い非コード RNA (lncRNA) の RNA ターゲットを識別することができます。自家製、設計されたアンチセンス DNA のオリゴヌクレオチドのプローブ適切に固定ティッシュまたは細胞のこの lncRNA に固有の交配に基づいて、それは効率的に、lncRNA のすべての RNA ターゲットの捕獲をできます。
10:53
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
関連ビデオ
9018 Views
11:19
Novel RNA-Binding Proteins Isolation by the RaPID Methodology
8943 Views
10:52
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
8091 Views
11:00
Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
13769 Views
09:45
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
11028 Views
07:24
Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
2396 Views
13:00
A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
11726 Views
07:55
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
3589 Views
13:34
Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
27534 Views
09:36
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
25.3K Views
Read Article
Cite this Article
Torres, M., Becquet, D., Guillen, S., Boyer, B., Moreno, M., Blanchard, M., Franc, J., François-Bellan, A. RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA. J. Vis. Exp. (134), e57379, doi:10.3791/57379 (2018).
Download .ris file
Copy
Share Video
.