Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

Richard B. McCosh; Michael J. Kreisman; Kellie M. Breen

doi:10.3791/57894

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

拍動性の黄体形成ホルモンの分泌の評価のためマウス尾先端採血を頻繁に

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05:58 min

July 4, 2018

DOI: 10.3791/57894-v

Richard B. McCosh¹, Michael J. Kreisman¹, Kellie M. Breen¹

¹Department of Reproductive Medicine,University of California, San Diego School of Medicine

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Overview

This study describes a tail-tip blood sampling protocol for the collection of blood samples in unrestrained mice, focusing on the measurement of pulsatile luteinizing hormone (LH) secretion. The method is designed to enable reliable assessments of LH pulsatile secretion, contributing to research in reproductive neuroendocrinology.

Key Study Components

Area of Science

Neuroendocrinology
Reproductive biology
Animal behavior

Background

Luteinizing hormone pulsatile secretion is critical in reproductive physiology.
Reliable blood sampling techniques are essential for accurately measuring hormonal fluctuations.
Existing methods may not adequately account for the effects of animal stress.
This protocol aims to minimize mouse stress during blood collection.

Purpose of Study

To develop a reliable tail-tip blood sampling protocol for unrestrained mice.
To assess LH pulsatile secretion in response to both acute and chronic treatments.
To improve the accuracy of neuroendocrinological research methods.

Methods Used

The protocol involves daily handling and habituation of mice prior to blood collection.
Adult female C57BL/6 mice were used, with blood collected from the tail tip for LH measurement.
Frequent handling sessions helped facilitate a stress-free environment.
Blood samples were collected over a set timeframe to establish hormonal baselines.

Main Results

The technique led to successful collection of LH levels in control animals.
Psychosocial stress from restraint significantly suppressed LH secretion in experimental groups.
Stress minimized reliable measurements of pulsatile LH secretion, highlighting the method's efficacy.
Environmental disturbances were found to affect hormonal measurements.

Conclusions

This study establishes a novel method for obtaining accurate hormonal measurements in mice.
The protocol allows researchers to explore LH regulation effectively in neuroendocrinology studies.
Understanding the impact of stress on hormonal secretion contributes to broader insights into reproductive physiology and animal behavior.

Frequently Asked Questions

What are the advantages of this blood sampling protocol?

This protocol minimizes stress by allowing mice to remain unrestrained, facilitating more reliable hormonal measurements.

How is the tail sample obtained?

Blood is collected by gently clipping the tail tip and stroking it to form a droplet, which is then sampled.

What is the biological model used in this study?

The study uses adult female C57BL/6 mice, which are well-characterized for neuroendocrinology research.

How does this method contribute to neuroendocrinology?

By providing reliable blood sampling under unrestrained conditions, researchers can better understand LH pulsatile secretion and its regulation.

What are key considerations when using this method?

Minimizing environmental disturbances is crucial, as noise and interruptions can impact hormonal measurements.

Can this method be adapted for other experiments?

Yes, the protocol can be adapted to assess other circulating factors, broadening its applicability in research.

ここで気ままなマウスで頻繁にサンプルコレクションの尻尾先端血液サンプリングプロトコルを提案します。このメソッドは、拍動性の黄体形成ホルモンの分泌の評価パターンに対して有用です、他の循環要因の分析のために合わせることができます。

頻繁な尾端採血の全体的な目標は、覚醒していない麻酔をかけられた正常な行動マウスのLHと呼ばれる拍動性黄体形成ホルモン分泌を測定するための全血サンプルを取得することです。この方法は、マウスの黄体形成ホルモンの拍動分泌を評価するための信頼性の高いプロトコルを提供することにより、再現性のある神経内分泌学の分野における重要な質問に答えるのに役立ちます。この技術の主な利点は、頻繁な取り扱いと慣れにより、急性または慢性の実験的治療後の堅牢で信頼性の高い黄体形成ホルモンの脈拍測定が保証されることです。

採血前の5週間は、月曜日から金曜日まで毎日マウスを取り扱います。最後の2週間は、土曜日と日曜日にもマウスを扱います。マウスの取り扱いは、バイオセーフティキャビネットで行ってください。

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神経科学問題 137 マウス血液サンプル尾先端頻繁にサンプリングレート黄体形成ホルモンホルモンパルス