11,478 Views
•
09:14 min
August 20, 2018
DOI:
10.3791/58283-v
ここでは、昆虫にセルとバキュロ ウイルス蛋白質結晶化の植物の分泌タンパク質の大量生産にタンパク質発現のシステムを利用するためのプロトコルを提案する.バキュロ ウイルス発現ベクターは、GP67 や植物タンパク質分泌発現昆虫細胞での昆虫ヘモリン シグナルペプチドと変更されています。
11:12
Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
関連ビデオ
12343 Views
06:11
Production and Purification of Baculovirus for Gene Therapy Application
14950 Views
08:12
Expression and Purification of Mammalian Bestrophin Ion Channels
8304 Views
12:18
Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System
24072 Views
08:57
Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
7325 Views
13:51
The MultiBac Protein Complex Production Platform at the EMBL
15921 Views
07:50
Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
49096 Views
23:21
Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana
41427 Views
13:52
Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
23362 Views
09:11
A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems
23495 Views
Read Article
Cite this Article
Chakraborty, S., Trihemasava, K., Xu, G. Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells. J. Vis. Exp. (138), e58283, doi:10.3791/58283 (2018).
Download .ris file
Copy
Share Video
.