実用的で実現可能な方法のセットを使用してRAFファミリーキナーゼの疾患関連変異体を特徴付けます
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07:49 min
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July 17, 2019
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概要
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本稿では、インビトロキナーゼアッセイ、RAF共活性化アッセイ、および相補的なスプリットルシフェラーゼアッセイを含む、RAFファミリーキナーゼの疾患関連変異体を特徴付けるための実用的かつ実行可能な方法のセットを提示した。
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Yap, J., Yuan, J., Tee, Z. H., Huang, X., Ng, W. H., Hu, J. Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods. J. Vis. Exp. (149), e59795, doi:10.3791/59795 (2019).
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