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Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry
JoVE Journal
医学
This content is Free Access.
JoVE Journal 医学
Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

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08:23 min

November 05, 2019

DOI:

08:23 min
November 05, 2019

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概要

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Here, we present oxygen gradient ektacytometry, a rapid and reproducible method to measure red blood cell deformability in samples from patients with sickle cell disease under controlled deoxygenation and reoxygenation. This technique provides a way to study red blood cell sickling and to monitor sickle cell disease treatment efficacy.

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