Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

Mingjun Xie; Kang Yu; Yuan Sun; Lei Shao; Jing Nie; Qing Gao; Jingjiang Qiu; Jianzhong Fu; Zichen Chen; Yong He

doi:10.3791/60545

ゼラチンメタクリロイルヒドロゲルベースバイオインクの3Dバイオプリンティングのプロトコル

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Bioengineering

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JoVE Journal Bioengineering

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

ゼラチンメタクリロイルヒドロゲルベースバイオインクの3Dバイオプリンティングのプロトコル

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10:25 min

December 21, 2019

DOI: 10.3791/60545-v

Mingjun Xie^1,2, Kang Yu^1,2, Yuan Sun^1,2, Lei Shao^1,2, Jing Nie^1,2, Qing Gao^1,2, Jingjiang Qiu³, Jianzhong Fu^1,2, Zichen Chen^1,2, Yong He^1,2

¹State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering,Zhejiang University, ²Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering,Zhejiang University, ³School of Mechanics and Safety Engineering,Zhengzhou University

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Overview

This article presents a method for the 3D bioprinting of gelatin methacryloyl (GelMA). It discusses strategies to enhance the printability of GelMA, which is crucial for various biomedical applications.

Key Study Components

Area of Science

3D Bioprinting
Biomaterials
Biomedical Engineering

Background

GelMA hydrogels are gaining popularity in 3D bioprinting.
Low viscosity of GelMA limits its printability.
Strategies are needed to improve its application in biomedical fields.
Potential applications include organ damage therapy.

Purpose of Study

To share effective printing strategies for GelMA.
To explore the special properties of GelMA for various 3D structures.
To contribute to the development of biomedical applications.

Methods Used

Utilization of GelMA's unique properties.
Development of various printing methods.
Strategies for embedding or tracing printed structures.
Addressing challenges in dissolving freeze-dried GelMA.

Main Results

Demonstrated effective printing techniques for GelMA.
Showcased potential applications in therapy.
Identified challenges in the dissolution of GelMA.
Provided insights for further research in 3D bioprinting.

Conclusions

Improved methods for 3D bioprinting of GelMA can enhance biomedical applications.
Further research is needed to optimize GelMA's printability.
Potential for significant contributions to organ therapy.

Frequently Asked Questions

What is GelMA?

GelMA is a gelatin-based hydrogel used in 3D bioprinting.

Why is viscosity important in 3D bioprinting?

Viscosity affects the printability and structural integrity of the printed material.

What are the applications of GelMA?

GelMA can be used in tissue engineering and regenerative medicine.

What challenges are associated with GelMA?

Dissolving freeze-dried GelMA can be time-consuming and difficult.

How can GelMA structures be utilized in therapy?

They can be injected or used to trace damaged organs for therapeutic purposes.

What is the significance of 3D bioprinting?

3D bioprinting allows for the creation of complex tissue structures for research and medical applications.

ここで提示するゼラチンメタクリロイルの3Dバイオプリンティングのための方法である。

GelMA ヒドロゲルベースのバイオニクスは、3D バイオプリンティングで非常に人気があります。しかし、低粘度は、その印刷可能性を制限します。ここでは、他の研究者と私たちの研究室でGelMAを印刷するための戦略を共有しています。

このビデオでは、この生体材料の特殊な特性を十分に活用し、さらに生物医学の応用に貢献できる異なる3D構造のためのいくつかの印刷方法を提案する。例えば意味を示す臓器損傷を取るためには、埋め込みまたは印刷されたGelMA構造をトレースすることによって対応する治療に使用される可能性がある。凍結乾燥したGelMAを短時間で完全に溶解することは容易ではありません。

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