生体材料に対する細胞毒性と細胞応答へのアクセス

4.6K 回視聴

15 件の引用

09:46 分

2021年7月8日

10.3791/61512-v

2021年7月8日

4.6K 回視聴

Anabela Baptista Paula^*¹^,²^,³^,⁴ , Mafalda Laranjo^*²^,³^,⁴^,⁵ , Ana Sofia Coelho¹^,²^,³^,⁴ , Ana Margarida Abrantes²^,³^,⁴^,⁵ , Ana Cristina Gonçalves²^,³^,⁴^,⁶ , Ana Bela Sarmento-Ribeiro²^,³^,⁴^,⁶ , Manuel Marques Ferreira²^,³^,⁴^,⁷ , Maria Filomena Botelho²^,³^,⁴^,⁵ , Carlos Miguel Marto¹^,²^,³^,⁴^,⁸ , Eunice Carrilho¹^,²^,³^,⁴

¹Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, ²Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), University of Coimbra, Coimbra, Portugal, ³Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, Portugal, ⁴Clinical Academic Center of Coimbra, CACC, Portugal, ⁵Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, ⁶Laboratory of Oncobiology and Hematology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, ⁷Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, ⁸Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal

^* These authors contributed equally

この方法論は、フローサイトメトリー、RT-PCR、免疫細胞化学、およびその他の細胞および分子生物学的手法を含む生存率アッセイおよび表現型分析を使用して、可溶性抽出物の調製を通じて生体材料の細胞毒性を評価することを目的としています。

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Chapters in this video

0:05

Introduction

1:12

Pellet Preparation and Soluble Biomaterial Extracts Acquisition

2:11

Morphology Evaluation

3:28

Cell Function Assessment

5:54

Mineralized Formation Assessment

6:50

Results: Cell Viability, Morphology, and Protein Expression in Response to Biomaterial Exposure

8:30

Conclusion

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