異種移植モデルの血液中の循環性豚特異的DNAの定量化
3.4K 回視聴
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2 件の引用
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07:34 分
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2020年9月22日
DOI :10.3791/61579-v
2020年9月22日
•3.4K 回視聴
Yangyang Deng*1,2 , Ming Zhou*1,3 , Ying Lu1 , Jiao Chen1 , Zuhui Pu4 , Dongjing Yu1,5 , Yifan Dai6 , Yongqiang Zhan7 , Lisha Mou1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Xianning Hospital of Traditional Chinese Medicine, 3Liver-biotechnology (Shenzhen) Co., Ltd., 4Department of Radiology, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 5Department of Life Sciences, University of Toronto, 6Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 7Department of Hepatopancreatobiliary Surgery, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital
* These authors contributed equally
本プロトコルでは、ブタ特異的プライマーを設計し、プラスミド含有ブタ特異的DNA断片を構築し、定量のための標準的な曲線を確立した。種特異的プライマーを用いて、cpsDNAを、豚からマウスへの細胞移植モデルおよびブタからサルへの動脈パッチ移植モデルにおいてqPCRによって定量化した。
Chapters in this video
0:04
Introduction
0:52
Isolating Genomic DNA
2:20
Verification of Primer Specificity
3:42
Standard Curve of cpsDNA
4:45
Quantitation of Circulating Pig-specific DNA
5:55
Results: PCR Validation of Porcine Specific Primers
6:51
Conclusion
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