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JoVE Journal
Environment
卵殻を使わない孵化による小可塑性の微生物の発生毒性による鳥胚発生への影響
卵殻を使わない孵化による小可塑性の微生物の発生毒性による鳥胚発生への影響
JoVE Journal
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JoVE Journal Environment
Ecotoxicological Effects of Microplastics on Bird Embryo Development by Hatching without Eggshell

卵殻を使わない孵化による小可塑性の微生物の発生毒性による鳥胚発生への影響

Full Text
5,125 Views
08:11 min
August 14, 2021

DOI: 10.3791/61696-v

Liyi Wang1,2, Nana Xue1,2, Wenfeng Li2, Rehemanjiang Wufuer1, Daoyong Zhang1,3

1Xinjiang Key Laboratory of Environmental Pollution and Bioremediation, Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences, 2University of Chinese Academy of Sciences, 3Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment,Zhejiang University of Technology

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Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This paper introduces a method of hatching without using an eggshell for toxicological studies of particle pollutants such as microplastics.

Key Study Components

Area of Science

  • Toxicology
  • Environmental Science
  • Pollution Studies

Background

  • Microplastics are pervasive environmental pollutants.
  • Traditional hatching methods may not be suitable for certain studies.
  • Innovative methods are needed to assess the impact of pollutants.
  • This study explores an alternative hatching technique.

Purpose of Study

  • To develop a method for hatching without an eggshell.
  • To facilitate toxicological assessments of microplastics.
  • To improve the reliability of experimental results in pollution studies.

Methods Used

  • Development of a novel hatching technique.
  • Application of the method to toxicological studies.
  • Assessment of particle pollutants.
  • Comparison with traditional hatching methods.

Main Results

  • The new hatching method was successful in producing viable subjects.
  • Results indicated that microplastics can be effectively studied using this approach.
  • Findings suggest improved outcomes compared to traditional methods.
  • The method shows promise for future toxicological research.

Conclusions

  • The method of hatching without an eggshell is viable.
  • This approach enhances the study of particle pollutants.
  • Future research can build on these findings to explore further applications.

Frequently Asked Questions

What are microplastics?
Microplastics are small plastic particles that can pollute the environment and pose risks to wildlife.
Why is traditional hatching not suitable for toxicological studies?
Traditional hatching methods may interfere with the assessment of pollutants or not provide the necessary conditions for study.
How does the new method improve toxicological studies?
The new method allows for more controlled conditions and reduces interference from the eggshell.
What implications do the findings have for environmental science?
The findings suggest that new methods can enhance our understanding of the effects of pollutants on ecosystems.
Can this method be applied to other pollutants?
Yes, the method may be adaptable for studying various types of environmental pollutants.

本論文では、マイクロプラスチックなどの粒子汚染物質の毒物学的研究に卵殻を用いずに孵化する方法を紹介する。

この実験は4つの部分を囲みます。露光前の準備、殻を含まないウズラの卵の孵化、インキュベーションプロセスおよびサンプル採取、および代表的な結果。具体的な実験プロセスについては、以下のビデオをご覧ください。暴露試験では、ウズラ受精卵の同じバッチを実験のために選択した。同様の大きさの卵を選択し、実験誤差を減らした。同時に、すべてのウズラは卵を受精させ、卵は完全に洗浄する必要があります。その後、孵化前、受精したウズラの卵、および使用される卵はペニシリンとマイシンで殺菌された置くとき、上面を下に注意する必要があり、その後鈍い端が上を向く必要があります。卵のクリーニングプロセスはビデオに表示されません。次のステップは、卵の殻を接地するプロセスです。それは準備の最も重要なステップです。唱えられる卵の殻は、歯科ドリルでセンターエッグサイズのカットですが、壊れることはできません。切開の破壊面を十分に滑らかにしようと、インキュベーターは75%アシノール溶液で解毒した。湿度60%と38の保育器に置かれたウズラの受精卵

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