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An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants
JoVE Journal
生物学
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JoVE Journal 生物学
An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants

An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants

2,349 Views

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06:09 min

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March 09, 2021

DOI:

10.3791/62287-v

DOI:
10.3791/62287-v

•

06:09 min
March 09, 2021

•

14 Views
, , , , , ,
1Institute for Bioscience and Biotechnology Research,University of Maryland, 2Department of Nutrition and Food Science,University of Maryland College Park, 3Department of Plant Sciences and Landscape Architecture,University of Maryland College Park

概要

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We present a protocol for constructing a simple spore-distribution system consisting of an inoculation box with a ~50 µm mesh and a transparent plastic chamber. This can be used to evenly inoculate plants with powdery mildew spores, thereby enabling accurate and reproducible assessment of disease phenotypes of plants under study.

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Cite this Article

Wu, Y., Diaz, D., Yin, J., Bloodgood, D., Sexton, W., Wei, C., Xiao, S. An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants. J. Vis. Exp. (169), e62287, doi:10.3791/62287 (2021).

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