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14:54 min
February 12, 2022
DOI:
10.3791/62438-v
マウス肝細胞から長期 の外ビトロ 3Dオルガノイド培養システムが確立された。これらのオルガノイドは、shRNA/異所構造、siRNAトランスフェクションおよびCRISPR-Cas9エンジニアリングのレンチウイルス感染によって継代され、遺伝的に操作することができる。
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Lian, J., Meng, X., Zhang, X., Hu, H. Establishment and Genetic Manipulation of Murine Hepatocyte Organoids. J. Vis. Exp. (180), e62438, doi:10.3791/62438 (2022).
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