エクソソームベースのドーパミンキャリアシステムの定式化と特性評価
3.6K 回視聴
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6 件の引用
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06:08 分
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2022年4月4日
DOI :10.3791/63624-v
2022年4月4日
•3.6K 回視聴
Burçak Yavuz1,2 , Hakan Darici3,4,5,6 , Aslı Pınar Zorba Yildiz2,7 , Emrah Şefik Abamor2 , Murat Topuzoğullari2 , Melahat Bağirova8 , Adil Allahverdiyev8 , Erdal Karaoz3,4,5,6,9
1Vocational School of Health Services/İstanbul, Altinbas University, 2Chemical Metallurgy Faculty, Department of Bioengineering, Yildiz Technical University, 3Faculty of Medicine, Department of Histology & Embryology, Istinye University, 43D Bioprinting Design & Prototyping R&D Center, Istinye University, 5Stem Cell and Tissue Engineering R&D Center, Istinye University, 6Medicine Faculty, Istinye University, 7Vocational School of Health Services, Istinye University, 8The V. Akhundov National Scientific Research Medical Prophylactic Institute, 9Center for Stem Cells and Regenerative Medicine (LivMedCell), Liv Hospital
ここでは、ウォートンゼリー間葉系幹細胞から幹細胞の単離エクソソームをドーパミン負荷することで製剤を得ることを目指しました。エキソソームの単離と特性評価、得られたエクソソームへの薬物負荷、および開発された製剤の細胞毒性活性は、このプロトコルで説明されています。
Chapters in this video
0:04
Introduction
0:56
Culturing and Cryopreservation of Wharton's Jelly Mesenchymal Stem Cells
1:30
Production of Exosomes from Wharton's Jelly Mesenchymal Stem Cells
2:20
Dopamine Loading into Exosomes and Characterization of Dopamine Loaded Exosomes
3:15
Results: Development of a Nanocarrier System by Encapsulation of Dopamine into Exosomes Isolated from WJ‐MSCs
5:07
Conclusion
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