液体および氷中のウイルス集合体の高解像度イメージングの進歩

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6 件の引用

08:31 分

2022年7月20日

10.3791/63856-v

2022年7月20日

3.2K 回視聴

Liza-Anastasia DiCecco^*¹^,² , Samantha Berry^*¹ , G. M. Jonaid^*¹^,³ , Maria J. Solares¹^,⁴ , Liam Kaylor¹^,⁴ , Jennifer L. Gray⁵ , Carol Bator⁶ , William J. Dearnaley¹ , Michael Spilman⁷ , Madeline J. Dressel-Dukes⁸ , Kathryn Grandfield² , Sarah M. McDonald Esstman⁹ , Deborah F. Kelly¹^,⁵^,⁶

¹Department of Biomedical Engineering, Pennsylvania State University, ²Department of Materials Science and Engineering, McMaster University, ³Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, ⁴Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, ⁵Materials Research Institute, Pennsylvania State University, ⁶Huck Institutes of the Life Sciences, Pennsylvania State University, ⁷Applications team, Direct Electron, ⁸Application Scientist, Protochips, Inc., ⁹Department of Biology, Wake Forest University

^* These authors contributed equally

ここでは、透過型電子顕微鏡を使用してナノスケールでの液体EMおよびクライオEM分析に適したウイルスアセンブリを調製するためのプロトコルについて説明します。

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SARS CoV 2

Chapters in this video

0:05

Introduction

1:43

Loading the Specimen Holder for Liquid-EM

2:47

Production of Microchip Sandwich Assemblies

3:51

Imaging Specimens Using a Transmission Electron Microscope

5:15

Analysis of SARS-CoV-2 Sub-Viral Assemblies in Liquid

6:09

Results: Representative structures of AAV, SARS-CoV-2 Sub-Viral Assemblies and Rotavirus DLPs Prepared in Liquid and Vitreous Ice Using the Microchip Sandwich Technique

7:24

Conclusion