A Mice Model of Chlorhexidine Gluconate-Induced Peritoneal Damage

Min-Yu Chang; Hsi-Hao Wang; Li-Hung Chen; Jhen Gao; Shih-Yuan Hung; Yuan-Yow Chiou; Yi-Che Lee

doi:10.3791/63903

JoVE Journal
Medicine

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Medicine

A Mice Model of Chlorhexidine Gluconate-Induced Peritoneal Damage

グルコン酸クロルヘキシジンによる腹膜損傷のマウスモデル

Full Text

2,323 Views

04:25 min

April 28, 2022

DOI: 10.3791/63903-v

Min-Yu Chang*^1,2, Hsi-Hao Wang*^1,2, Li-Hung Chen*¹, Jhen Gao*¹, Shih-Yuan Hung^1,2, Yuan-Yow Chiou^3,4, Yi-Che Lee^1,2,5

¹School of Medicine, College of Medicine,I-Shou University, ²Division of Nephrology, Department of Internal Medicine,E-DA Hospital, ³Department of Pediatrics,National Cheng Kung University Hospital, ⁴Institute of Clinical Medicine, College of Medicine,National Cheng Kung University, ⁵Division of Nephrology, Department of Internal Medicine,E-DA Dachang Hospital

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This protocol establishes a peritoneal dialysis (PD) mouse model of chlorhexidine gluconate (CG)-induced peritoneal fibrosis. The model is simple and easy to use compared to other PD animal models.

Key Study Components

Area of Science

Neuroscience
Biology

Background

Establishing animal models for peritoneal dialysis is crucial for research.
The model aims to improve PD therapy technology and efficacy.
It helps in studying physiological and pathological changes during PD therapy.

Purpose of Study

To create a reliable PD mouse model.
To facilitate research on peritoneal fibrosis.
To enhance understanding of PD therapy outcomes.

Methods Used

Dilution of 0.1% chlorhexidine gluconate in 15% ethanol.
Demonstration of the procedure by a research assistant.
Utilization of a simple and visible model for ease of use.
Comparison with other peritoneal dialysis animal models.

Main Results

Establishment of a straightforward PD mouse model.
Improved visibility and usability compared to existing models.
Potential for enhanced research on peritoneal fibrosis.
Contribution to better understanding of PD therapy.

Conclusions

The PD mouse model is effective for studying peritoneal fibrosis.
It provides a foundation for future research in PD therapy.
Simple protocols can lead to significant advancements in the field.

Frequently Asked Questions

What is peritoneal dialysis?

Peritoneal dialysis is a treatment for kidney failure that uses the lining of the abdomen to filter waste from the blood.

Why is a mouse model used in this study?

Mouse models are commonly used in research due to their genetic similarity to humans and the ability to control experimental conditions.

What is chlorhexidine gluconate?

Chlorhexidine gluconate is an antiseptic used for skin disinfection and to sterilize surgical instruments.

How does this model improve PD therapy?

This model allows researchers to study the effects of PD therapy on peritoneal fibrosis, potentially leading to improved treatment methods.

Who demonstrated the procedure?

The procedure was demonstrated by Fang-Ling Liao, a research assistant from the laboratory.

本プロトコルは、グルコン酸クロルヘキシジン(CG)誘発腹膜線維症の腹膜透析(PD)マウスモデルを確立する。現在のモデルは、他のPD動物モデルと比較してシンプルで使いやすいです。

腹膜透析の動物モデルPDの確立は非常に重要です。PD療法の技術と有効性を改善し、最終的には患者の生存率を向上させることができることを願っています。この腹膜透析マウスモデルは、他の腹膜透析動物モデルと比較して、シンプルで使いやすく、目に見える。

適切な腹膜透析動物モデルは、研究者がPD療法中の腹膜、生理学的、および病理学的変化を研究するのに役立ちます。手順のデモンストレーションは、私の研究室の研究助手であるFang-Ling Liaoです。まず、0.1%グルコン酸クロルヘキシジンを15%エタノールで希釈して化学刺激剤を調製します。

View the full transcript and gain access to thousands of scientific videos

Explore More Videos

医学第182号グルコン酸クロルヘキシジン腹膜透析モデル腹膜線維症