Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography

Brikena Gusek; Robert Folk; Tala Curry; Mitra Esfandiarei

doi:10.3791/63918

テンソメトリック小容量チャンバーミオグラフィを用いたマウス胸部大動脈の内皮依存性血管弛緩の測定

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Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography

テンソメトリック小容量チャンバーミオグラフィを用いたマウス胸部大動脈の内皮依存性血管弛緩の測定

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09:47 min

August 12, 2022

DOI: 10.3791/63918-v

Brikena Gusek¹, Robert Folk¹, Tala Curry², Mitra Esfandiarei¹

¹Department of Biomedical Sciences, College of Graduate Studies,Midwestern University, ²Department of Child Health, College of Medicine-Phoenix,University of Arizona, Phoenix

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Overview

This protocol illustrates the application of the tensometric myograph technique for assessing mouse aortic endothelial function under controlled physiological conditions. The method is recognized for its reliability and reproducibility in studying vascular reactivity.

Key Study Components

Research Area

Vascular biology
Endothelial function assessment
Microscopic analysis of blood vessels

Background

The importance of endothelial function in vascular biology.
The role of tensometric myography in vascular reactivity studies.
Challenges in isolating and assessing particular layers of blood vessels.

Methods Used

Tensometric myography as the primary technique
Mouse aorta as the biological model
Use of HEPES-PSS solution and high-potassium solution for experimentation

Main Results

Demonstrated reproducibility of the measurements.
Successful isolation and assessment of aortic segments' function.
Enhanced understanding of acetylcholine-induced vasorelaxation dynamics.

Conclusions

The study validates the tensometric myograph technique for endothelial function research.
This method can significantly advance insights into vascular health and diseases.

Frequently Asked Questions

What is the significance of the tensometric myograph technique?

The tensometric myograph technique allows for precise measurement of vascular reactivity, which is crucial for understanding endothelial function.

How do researchers ensure the reliability of measurements?

Reliability is ensured through rigorous experimental protocols and by maintaining controlled physiological conditions throughout the assay.

What are the advantages of using mouse aorta in this research?

The mouse aorta serves as an accessible and relevant model for studying vascular biology and endothelial functions.

Can this technique be applied to other blood vessels?

Yes, the tensometric myograph technique can be adapted for use with other small resistance and large conduit blood vessels.

What role do vasoconstrictors play in this experiment?

Vasoconstrictors are used to pre-contract the aortic segments, allowing researchers to study subsequent vasorelaxation responses to various agents.

Is the technique suitable for high-throughput screening?

While the technique is robust, its detailed nature may limit the pace of high-throughput screenings compared to other methods.

What precautions are taken during the myographic measurements?

Precautions include maintaining optimal temperatures, aeration of solutions, and careful handling of aortic segments to prevent tissue damage.

本プロトコルは、マウス大動脈内皮機能の実験的 ex vivo 評価におけるマルチチャンバーミオグラフシステムを用いたテンソメトリックミオグラフ技術の概念および技術的適用を記載する。

アイソメトリックまたはテンソメトリックミオグラフィーを、適切に制御された生理学的条件で小さな抵抗または大きな導管血管の反応性を測定するためのゴールドスタンダードプロトコルとして実証します。測定は再現性と信頼性が高いです。この技術は、研究者が血管の異なる層を個別に研究したり、血管壁の異なる層間の相互作用を同時に調査したりすることを可能にする上で非常に貴重です。

手順を実演するのは、私の研究室の研究室スペシャリストであるロバートフォークです。まず、ウォーターバスをオンにして摂氏37度に設定します。適切にラベル付けされた2つのビーカーを水浴に入れ、1つは600ミリリットルのHEPES-PSS溶液、もう1つは150ミリリットルの高カリウム溶液を入れます。

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