Optimization of Transesophageal Atrial Pacing to Assess Atrial Fibrillation Susceptibility in Mice

Matthew B. Murphy; Kyungsoo Kim; Prince J. Kannankeril; Katherine T. Murray

doi:10.3791/64168

JoVE Journal
Biology

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JoVE Journal Biology

Optimization of Transesophageal Atrial Pacing to Assess Atrial Fibrillation Susceptibility in Mice

マウスにおける心房細動感受性評価のための経食道心房ペーシングの最適化

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08:05 min

June 29, 2022

DOI: 10.3791/64168-v

Matthew B. Murphy¹, Kyungsoo Kim¹, Prince J. Kannankeril¹, Katherine T. Murray¹

¹Departments of Medicine, Pediatrics and Pharmacology,Vanderbilt University School of Medicine

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Overview

This protocol describes the optimization of transesophageal atrial pacing to evaluate atrial fibrillation susceptibility in mice. The method allows for repeated testing in the same animal, enhancing the reproducibility of results.

Key Study Components

Research Area

Atrial fibrillation
Electrophysiology
Mouse models

Background

Understanding atrial fibrillation's electrophysiologic properties.
The importance of protocol standardization for experimental reproducibility.
Effects of pacing-induced parasympathetic stimulation on arrhythmias.

Methods Used

Transesophageal atrial pacing
Mice
ECG recording and analysis

Main Results

Optimal settings for pacing were established.
Data showed variations in AF susceptibility across different testing conditions.
Identified factors that could confound results, such as parasympathetic stimulation.

Conclusions

The study presents a refined protocol for assessing atrial fibrillation in mice.
It underlines the significance of meticulous protocol design in electrophysiological research.

Frequently Asked Questions

What is transesophageal atrial pacing?

It is a technique used to induce and study atrial fibrillation in experimental models, particularly in mice.

Why is it essential to optimize experimental parameters?

Optimizing parameters enhances the accuracy and reproducibility of research findings related to atrial fibrillation.

How can parasympathetic stimulation affect results?

It can lead to inadvertent induction of arrhythmias, confounding the interpretation of data regarding atrial fibrillation susceptibility.

What age should mice be for optimal testing?

Mice should be at least 12 weeks old for acquired models and start biweekly pacing at 8 weeks for genetic models.

What measures are taken to analyze atrial fibrillation?

Electrophysiologic parameters and duration of AF episodes are recorded, allowing for comprehensive analysis of susceptibility.

Why is serial testing important?

It allows researchers to assess changes in AF susceptibility over time in the same animal, improving data integrity.

Is this method applicable to genetic studies?

Yes, it is specifically designed to assess both genetic and acquired models of atrial fibrillation.

本プロトコルは、マウスにおける心房細動感受性を評価するために経食道心房ペーシングを使用する場合の実験パラメータの最適化を記載する。

経食道心房ペーシングを使用したプログラムされた電気刺激は、マウスの電気生理学的特性と心房細動に対する感受性を決定することができます。ここでは、再現性を最適化できるプロトコル開発の標準化された手順について説明しました。心房ペーシングのための経食道アプローチは、典型的には生存手順であり、したがって、同じ動物での連続検査を可能にする。

プロトコル開発の重要なコンポーネントには、研究中のモデルの実験パラメータを最適化するための初期パレット研究と、ペーシング誘発副交感神経刺激によって引き起こされる不整脈の特定が含まれます。遺伝子モデルの場合、マウスを隔週の心房ペーシングにかけ、8週齢から開始してAF感受性の最適期間を決定し、一方がAF表現型を発症しない場合があるので男女ともに研究する。獲得モデルについては、マウスが身体的成熟を達成した後にペーシングを実施します。

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