Simple Continuous Glucose Monitoring in Freely Moving Mice

Doron Kleiman; Mika Littor; Mahmoud Nawas; Rachel Ben-Haroush Schyr; Danny Ben-Zvi

doi:10.3791/64743

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Biology

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Simple Continuous Glucose Monitoring in Freely Moving Mice

自由に動くマウスにおける簡単な連続グルコースモニタリング

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03:25 min

February 24, 2023

DOI: 10.3791/64743-v

Doron Kleiman¹, Mika Littor^1,2, Mahmoud Nawas³, Rachel Ben-Haroush Schyr¹, Danny Ben-Zvi¹

¹Department of Developmental Biology and Cancer Research, Institute of Medical Research Israel-Canada,The Hebrew University-Hadassah Medical School, ²Department of Military Medicine and “Tzameret”, Faculty of Medicine,Hebrew University of Jerusalem, and Medical Corps, Israel Defense Forces, ³Department of Surgery,Hadassah Medical Center-Ein Kerem Campus

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Overview

This study presents a straightforward method for implanting a commercial continuous glucose monitor (CGM) in mice to track glucose levels. The technique offers significant advantages, eliminating the need for complex microsurgery or bleeding, thus facilitating studies in obesity and diabetes models.

Key Study Components

Research Area

Diabetes research
Metabolic phenotype identification
Glucose monitoring in animal models

Background

Importance of monitoring glucose levels in obesity and diabetes research
Challenges associated with traditional surgical approaches
Need for effective methods to explore glucose management phenotypes

Methods Used

The procedure for inserting a CGM cannula
Mice as the biological model system
Utilization of suture techniques and sensor activation

Main Results

The method successfully secured the CGM cannula for extended monitoring
Data collected revealed new insights into metabolic phenotypes
Demonstrated feasibility and efficiency in data acquisition from mice

Conclusions

The study illustrates a reliable protocol for glucose monitoring in mice
This method contributes to advancing research in metabolic disorders

Frequently Asked Questions

What is the main advantage of this glucose monitoring method?

It simplifies the monitoring process and eliminates the need for invasive surgery or bleeding, making it less stressful for the mice.

How long can the CGM monitor glucose levels?

The method allows for continuous data collection, with effective monitoring over multiple days, as demonstrated in a three-day data collection period.

What biological models can benefit from this technique?

The technique is particularly useful in mouse models of obesity and diabetes, aiding in the identification of glucose management phenotypes.

What preparations are necessary before implanting the CGM?

It involves shaving the midline of the mouse's back, using depilatory cream, and making a small incision for cannula placement.

Are there any specific challenges when implementing this method?

The critical step is the careful insertion of the cannula and securing it properly to ensure it remains in place during the monitoring period.

Can the data collection parameters be adjusted?

Yes, the scripts provided allow modulation of various parameters during data analysis.

What type of data does this method produce?

The method produces continuous glucose monitoring data, which can reveal metabolic insights in mouse models.

ここでは、患者向けに設計された市販の連続血糖モニターをマウスに移植する簡単な方法を説明し、結果を分析するためのスクリプトを提供します。

私たちのプロトコルは、マウスのグルコースレベルを監視するための簡単で安価な方法を提供します。この技術の主な利点は、困難な顕微手術を必要とせず、出血やマウスの取り扱いを伴わないことです。この方法は、肥満および糖尿病のマウスモデルにおけるとらえどころのないグルコース管理表現型を同定するために使用することができる。

このプロトコルを試みる間、最も重要なステップは、カニューレを皮膚の下に挿入し、カニューレを所定の位置に保つために内側の縫合糸を締めることです。麻酔をかけたマウスの背中の正中線を剃り始め、脱毛クリームを使用して完全な脱毛を確実にします。センサーを開いてロードします。

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