肺由来サンプルからの微小血管および大血管内皮細胞の単離および精製(英語)
3K 回視聴
•
3 件の引用
•
05:26 分
•
2023年2月3日
DOI :10.3791/64885-v
2023年2月3日
•3K 回視聴
Roberto Plebani1,2 , Alessandra D'Alessandro2 , Paola Lanuti2,3 , Pasquale Simeone2,3 , Massimiliano Cinalli1 , Ilaria Righi4 , Alessandro Palleschi4,5 , Matteo Mucci1,2 , Marco Marchisio2,3 , Francesco Cappabianca1 , Marina Camera6,7 , Felice Mucilli1 , Mario Romano1,2
1Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 2Center on Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, 3Department of Medicine and Aging Sciences, University “G. d’Annunzio” of Chieti-Pescara, 4U.O. di Chirurgia Toracica e dei Trapianti di Polmone, Fondazione IRCCS Ca’ Granda - Ospedale Maggiore Policlinico di Milano, 5Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti, Università degli Studi di Milano, 6Department of Pharmaceutical Sciences, Università degli Studi di Milano, 7Unit of Cell and Molecular Biology in Cardiovascular Diseases, Centro Cardiologico Monzino IRCCS
挑戦的ですが、肺内皮細胞の単離は肺炎症の研究に不可欠です。本プロトコルは、大血管内皮細胞および微小血管内皮細胞の高収率、高純度単離のための手順を記載する。
Chapters in this video
0:04
Introduction
0:45
Sample Preparation and Enzymatic Digestion
1:47
Recovery of the Digested Cells and Removal of Non-Endothelial Cells (EC) by Filtration
2:32
Sedimentation of Cell Clusters and Cell Seeding
3:16
Results: Purification and Characterization of Freshly Isolated HLMVECs
4:30
Conclusion
Related Videos
著作権 © 2026 MyJoVE Corporation. 無断転載を禁じます。