Exploring Independent Effects of Follicle-Stimulating Hormone <em>In Vivo</em> in a Mouse Model

Yanjing Guo; Wei Li; Yinghan Wang

doi:10.3791/65665

JoVE Journal
Biology

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JoVE Journal Biology

Exploring Independent Effects of Follicle-Stimulating Hormone In Vivo in a Mouse Model

マウスモデルにおける卵胞刺激ホルモンの in vivo での独立効果の探索

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05:32 min

August 11, 2023

DOI: 10.3791/65665-v

Yanjing Guo^1,2,3, Wei Li^2,3, Yinghan Wang²

¹The Affiliated Hospital of Qingdao University, ²Department of Anesthesiology,Shandong Provincial Hospital Affiliated to Shandong First Medical University, ³Department of Anesthesiology, Shandong Provincial Hospital, Cheeloo College of Medicine,Shandong University

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Overview

This study investigates the extragonadal actions of follicle-stimulating hormone (FSH) in the context of perimenopausal health, using an ovariectomized and FSH-treated mouse model. Findings reveal how FSH influences serum cholesterol levels and may impact cardiovascular risks associated with menopause.

Key Study Components

Research Area

Pathophysiology in perimenopausal women
Extragonadal effects of FSH
Cardiovascular disease risk factors

Background

Menopause is linked to dyslipidemia and increased cardiovascular disease risk.
FSH's role in lipid metabolism is not fully understood.
The OVF mouse model closely mimics early menopause, allowing for targeted research on FSH.

Methods Used

Ovariectomy and FSH injection in a mouse model
Use of C-57 black six mice to evaluate FSH effects
Histological and biochemical assays to monitor hormonal changes

Main Results

FSH increased serum cholesterol levels independently of aspirin.
Blocking FSH signaling was effective in preventing hypocholesterolemia.
Establishes FSH's role in the dyslipidemic profile during menopause.

Conclusions

This study highlights the significant effects of FSH beyond reproductive tissues, particularly regarding cholesterol metabolism.
The findings are relevant for understanding menopause-related health risks.

Frequently Asked Questions

What is the significance of FSH in perimenopausal health?

FSH appears to play a crucial role in cholesterol metabolism, which can significantly affect cardiovascular health in perimenopausal women.

How was the OVF mouse model developed?

The OVF mouse model was developed to closely mimic the hormonal changes during early menopause by ovariectomy and FSH treatment.

What experimental methods were used in this study?

The study employed ovariectomy, FSH injection, and various biochemical assays to monitor hormonal levels and physiological responses.

What are the major findings regarding FSH treatment?

FSH treatment was found to increase serum cholesterol levels, indicating its role in metabolic changes during menopause.

How does this study impact future research?

It opens avenues for further investigation into the extragonadal roles of FSH and its implications for cardiovascular health.

Is the OVF mouse model cost-effective?

Yes, the OVF mouse model is noted for its stability, low cost, and ease of operation, making it suitable for various research studies.

What implications does this research have for menopausal treatments?

The research suggests that targeting FSH signaling could be a potential therapeutic strategy for managing cardiovascular risks associated with menopause.

さまざまな性腺外組織および臓器における卵胞刺激ホルモン(FSH)は、複数の疾患の病因と関連しています。卵巣摘出術およびFSH処理マウスモデル(OVF)を使用して、FSHの性腺外作用を調べることができます。

私たちの研究は、閉経周辺期の女性の生理学的および病理学的変化に焦点を当てています。本研究では、どの病態生理学的変化がFHCと関連しているかを明らかにしようとしています。更年期障害は脂質異常症と心脳血管疾患のリスク増加と関連しています。

このマウスモデルを用いて、アスピリンとは無関係に卵胞刺激ホルモンが血清コレステロール値を上昇させることを見出しました。さらに、FSHシグナル伝達を遮断することで、低コレステロール血症を効果的に予防することができます。このマウスモデルは、血清FSHレベルの上昇と比較的安定したエストロゲンレベルを特徴とする閉経移行の初期段階を模倣しています。

このモデルには、安定性、低コスト、操作が簡単であるという利点があり、FSHの生殖腺外作用を調査する研究に適しています。