Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue

Marianna Gabrielle Almeida Galv&#227;o; Brisa Miranda Andrade Santos; Cah&#234; Moreira Aguiar; Adriana Bozzi

doi:10.3791/65722

マウス精巣上体脂肪組織からの間葉系幹細胞の単離、In vitro拡大、および特性評価

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Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue

マウス精巣上体脂肪組織からの間葉系幹細胞の単離、In vitro拡大、および特性評価

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04:53 min

January 12, 2024

DOI: 10.3791/65722-v

Marianna Gabrielle Almeida Galvão¹, Brisa Miranda Andrade Santos¹, Cahê Moreira Aguiar², Adriana Bozzi^1,2

¹Laboratory of Immunobiology, Department of Biological Sciences,State University of Santa Cruz - UESC, ²Department of Medicine,Afya Medical Sciences College

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Overview

This study focuses on the extraction, cultivation, and characterization of adipose tissue-derived stem cells (ADSCs) from the epididymal adipose tissue of Swiss mice. The findings highlight ADSCs' potential as a therapeutic tool, particularly in managing inflammation associated with diseases like Schistosoma mansoni infection.

Key Study Components

Research Area

Adipose-derived mesenchymal stem cells
Inflammation control
Therapeutic applications in infection

Background

High potential of adipose tissue as a source of stem cells
Represents an abundant and accessible source
Defines a protocol for meticulous collection and processing

Methods Used

Isolation and culture of ADSCs
Swiss mice as the biological model
Characterization techniques including staining and morphological assessment

Main Results

Demonstrated ADSCs' multi-lineage differentiation potential
Effective control of granulomatous reactions in Schistosoma mansoni infection
Characterization confirmed morphology and potential of ADSCs

Conclusions

This study provides a reliable protocol for ADSC extraction and characterization
Highlights the therapeutic promise of ADSCs in inflammatory diseases

Frequently Asked Questions

What are adipose-derived stem cells?

Adipose-derived stem cells (ADSCs) are multipotent stem cells found in adipose tissue, capable of differentiating into various cell types.

How were the stem cells extracted in this study?

Stem cells were extracted from the epididymal adipose tissue of Swiss mice following aseptic techniques and specific processing methods.

What diseases could benefit from ADSC therapy?

Potential applications include treatment for inflammatory diseases and tissue regeneration.

What is the significance of this research?

This research fills a gap in the techniques for isolating ADSCs and demonstrates their therapeutic potential in a disease model.

What methodologies were used for characterization?

Techniques included morphological assessments and staining methods to evaluate differentiation potential.

Can ADSCs be used in combination with other therapies?

Yes, the study indicates that ADSCs can be combined with other treatments, such as praziquantel, to improve therapeutic outcomes.

脂肪組織は、間葉系幹細胞の優れた供給源です。ここでは、スイスのマウス精巣上体脂肪組織からの脂肪組織由来幹細胞(ADSC)の段階的な抽出、培養、および特性評価をもたらします。

私の研究は、いくつかの疾患からの炎症を改善するための他の治療法に関連する、または関連しない新しいアプローチ治療として、脂肪由来の間葉系幹細胞に焦点を当てています。脂肪間葉系幹細胞をプラジカンテルと組み合わせて使用することで、マンソン住血吸虫感染症における肉芽腫反応が大幅に制御されることを実証し、この併用治療アプローチの利点を強調しました。私たちのプロトコルは、無菌条件下での精巣上体脂肪組織の細心の注意を払ったコレクションの研究ギャップに対処し、激しい振とうを伴う物理的処理、および脂肪由来幹細胞を利用するための最適な時間を特定します。

私たちのプロトコルには、特に間葉系幹細胞の分離のための豊富な利用可能性と潜在的な強度という大きな利点があります。さらに、間葉系幹細胞は、抗炎症作用や組織再生作用などの強力な治療特性をもたらします。

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