Captive Maintenance and Venom Extraction of <em>Tityus serrulatus</em> (Brazilian Yellow Scorpion) for Antivenom Production

Thiago M. Chiariello; Denise M. Candido; Ricardo N. Oliveira; Aline V. V. Auada; Fan Hui Wen

doi:10.3791/65737

抗毒素生産のための Tityus serrulatus (ブラジルの黄色いサソリ)の飼育下維持と毒抽出

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Biology

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JoVE Journal Biology

Captive Maintenance and Venom Extraction of Tityus serrulatus (Brazilian Yellow Scorpion) for Antivenom Production

抗毒素生産のための Tityus serrulatus (ブラジルの黄色いサソリ)の飼育下維持と毒抽出

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05:27 min

October 6, 2023

DOI: 10.3791/65737-v

Thiago M. Chiariello¹, Denise M. Candido¹, Ricardo N. Oliveira¹, Aline V. V. Auada¹, Fan Hui Wen¹

¹Bioindustrial Center,Instituto Butantan

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Overview

This study details protocols for the maintenance and venom extraction of Tityus serrulatus, the Brazilian yellow scorpion, aimed at supporting antivenom production for the Brazilian health system. The enhanced procedures allow for increased capacity in venom extraction while considering animal welfare.

Key Study Components

Research Area

Scorpion venom extraction
Antivenom production
Animal welfare in laboratory settings

Background

High demand for scorpion antivenom in tropical countries
The importance of neutralizing immunoglobulins for treatment
Need for effective husbandry practices

Methods Used

Electrical stimulation for venom extraction
Tityus serrulatus as the biological system
Detailed housing preparation and cleaning protocols

Main Results

Capacity to maintain 4,000 to 20,000 scorpions over 10 years
Exponential increase in venom extraction efficiency
Production of approximately 100,000 vials of antivenom annually

Conclusions

The protocols significantly improve venom production for antivenom
Emphasizes the importance of sustainable practices in biological research

Frequently Asked Questions

What is the significance of Tityus serrulatus venom?

Tityus serrulatus venom is crucial for producing antivenom, which is essential in treating venomous bites in humans.

How does the extraction process work?

The process involves electrical stimulation to extract venom while minimizing harm to the scorpions.

Why is animal welfare considered in this research?

The protocols are designed to maintain optimal living conditions for the scorpions while ensuring effective venom extraction.

How much venom can be harvested annually?

The facility can produce over 80 grams of liquid venom annually, translating to significant amounts for antivenom production.

What are the challenges faced in maintaining scorpions?

Challenges include ensuring proper habitat conditions and managing feeding regimens for large populations.

What role do horses play in this study?

Horses are immunized using the extracted venom to produce antibodies, which are used in antivenom creation.

Is this extraction method standard in the industry?

This method differs from others as it does not require removal of the telson, allowing for more frequent extractions.

ここでは、ブラジルの医療システムの需要を満たすために、その後のサソリの抗毒素生産に毒を提供することを目的として、大規模な Tityus serrulatus Lutz and Mello(ブラジルの黄色いサソリ)の飼育に成功した維持および毒抽出プロトコルを紹介します。

このプロトコルは中和のウマ配達されたimmunoglobulinsまたはantivenomsの生産で使用される大量の毒液の抽出のためのサソリを維持する方法を記述する。熱帯諸国では、ヒトの毒を治療するための要となるサソリの抗毒素の需要が高まっています。従って維持のプロシージャは動物福祉およびよい実験室の練習を考慮して捕獲でサソリを保つ、最適の自然条件を、再現する。

これと並行して、毒の抽出はサソリのテルソンの電気刺激に基づいており、動物への有害な影響は最小限に抑えられています。このプロトコルにより、10年間で4、000頭から20、000頭の範囲で飼育下で維持される動物の数を指数関数的に増やすことができました。同時に、毒液は馬に免疫を与えて血漿を得るために十分に得られました、これはおよそ100、1年ごとの反毒素の000のガラスびんで起因する製造工程のための出発物質である。

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