遠位中動脈閉塞法を用いたマウスにおける急性虚血性脳卒中の誘発

Overview

This study presents a detailed protocol for establishing a distal middle cerebral artery occlusion (dMCAO) model in C57BL/6J mice using transcranial electrocoagulation. It focuses on evaluating the resulting neurological behaviors and histopathological features observed post-occlusion.

Key Study Components

Area of Science

• Neurological behavior assessment
• Histopathological analysis
• Ischemic stroke modeling

Background

• The medial cerebral artery (MCA) is key in ischemic strokes in humans.
• Various MCA occlusion methods exist, but dMCAO offers advantages in terms of surgical invasiveness and survival rates.
• The model is beneficial for exploring long-term functional recovery.

Purpose of Study

• To generate a reliable experimental ischemic stroke model in rodents.
• To assess behavioral outcomes and histopathological changes post-stroke.
• To utilize the model for future studies on cognition impairment.

Methods Used

• Transcranial electrocoagulation was employed to induce dMCAO.
• C57BL/6J mice were used to study neurological changes following occlusion.
• Behavioral tests such as grip strength, pole test, adhesive removal, and cylinder test were utilized.
• Histological examination of brain tissue was conducted post-behavioral assessments.

Main Results

• Mice exhibited significant reductions in grip strength and increased descent times on various behavioral tests.
• Histological analysis showed disordered neuron arrangements and reduced cell density in the peri-infarct area.

Conclusions

• The study establishes a robust dMCAO model with high reproducibility for stroke research.
• This methodology will enhance understanding of structural and behavioral outcomes after ischemic stroke.
• Future applications could focus on cognitive impairments in post-stroke recovery.

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