Isolation and Analysis of Aortic Arch and Root Lesions in an Atherosclerotic Mouse Model

Yu Yan; Guofang Wang; Longhua Liu

doi:10.3791/67875

アテローム性動脈硬化症マウスモデルにおける大動脈弓および大動脈基部病変の単離と解析

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JoVE Journal Medicine

Isolation and Analysis of Aortic Arch and Root Lesions in an Atherosclerotic Mouse Model

アテローム性動脈硬化症マウスモデルにおける大動脈弓および大動脈基部病変の単離と解析

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07:49 min

February 14, 2025

DOI: 10.3791/67875-v

Yu Yan*¹, Guofang Wang*¹, Longhua Liu¹

¹School of Exercise and Health,Shanghai University of Sport

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Overview

This protocol provides a comprehensive methodology for the quantitative analysis of atherosclerotic plaques to facilitate more in-depth research into the mechanisms and progression of atherosclerosis. The study investigates atherosclerotic lesion development in LDLR knockout mice, detailing techniques for isolation, staining, and analysis.

Key Study Components

Area of Science

Neuroscience
Cardiovascular Biology
Pathology

Background

Atherosclerosis is a major contributor to cardiovascular diseases.
Understanding plaque composition is crucial for assessing disease severity.
LDLR knockout mice serve as a model for studying atherosclerosis.
Histological examination is a key method for analyzing plaques.

Purpose of Study

To provide a detailed protocol for analyzing atherosclerotic plaques.
To assess the severity and composition of atherosclerosis in a mouse model.
To enhance understanding of atherosclerosis mechanisms.

Methods Used

Isolation and staining of atherosclerotic plaques.
Histological examination of lesions.
Assessment of biochemical markers.
Vascular ultrasound techniques.

Main Results

Detailed methodology for plaque analysis was established.
Technical challenges in dissection procedures were identified.
Comprehensive assessment techniques were outlined.
Findings contribute to understanding atherosclerosis progression.

Conclusions

The protocol aids in the quantitative analysis of atherosclerotic plaques.
Improved techniques can enhance research into cardiovascular diseases.
Further studies are needed to explore underlying mechanisms.

Frequently Asked Questions

What is the significance of studying atherosclerosis?

Atherosclerosis is a leading cause of cardiovascular diseases, making its study crucial for developing effective treatments.

How does the LDLR knockout mouse model contribute to this research?

LDLR knockout mice are used to mimic human atherosclerosis, allowing for detailed study of plaque development and progression.

What techniques are used for plaque analysis?

Techniques include histological examination, biochemical marker assessment, and vascular ultrasound.

What are the main challenges in the dissection procedure?

The precision required for in vivo aortic stripping poses significant technical hurdles.

What are the expected outcomes of this study?

The study aims to provide a comprehensive methodology for analyzing atherosclerotic plaques and understanding their progression.

How can this research impact cardiovascular disease treatment?

By improving understanding of atherosclerosis, this research can lead to better prevention and treatment strategies for cardiovascular diseases.

このプロトコルは、アテローム性動脈硬化症のプラークの定量分析のための包括的な方法論を提供し、アテローム性動脈硬化症のメカニズムと進行に関するより詳細な研究を促進します。

この研究では、LDLRノックアウトマウスの大動脈弓と根のアテローム性動脈硬化症病変の発達を調査し、アテローム性動脈硬化症の重症度プラーク組成を評価するための分離、染色、および分析のためのプロトコルを提供します。この研究で使用された組織学的検査に加えて、アテローム性動脈硬化症を評価するための実用的な技術には、生化学的マーカー、分子生物学的検査、および血管超音波も含まれます。解剖手順の精度は、アテローム性動脈硬化症のマウスモデル内でin vivo大動脈ストリッピングを行う際に最大の技術的ハードルをもたらします。

まず、安楽死させたマウスを仰臥位にして、1〜2層の吸収紙で覆われた発泡スチロールボードの上に置きます。手足をピンで固定して体を安定させます。マウスの腹部に75%エタノールをスプレーして、毛皮をきれいにし、湿らせます。

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