Name: Chromobacterium violaceumにおけるビオラセインの定量と生理活性化合物による阻害 (動画) | JoVE Journal
Uploaded: 2025-08-08T14:10:00.000+00:00
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Description: 学ぶChromobacterium violaceumにおけるクォラムセンシング阻害とビオラセイン定量法。ファルネソールやリナロールなどの生理活性化合物を用いて、細菌コミュニケーションを標的とした新規抗病原性戦略の開発に貢献します。

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<table><tbody><tr><td>DMSO&nbsp;</td><td>シンセ</td><td></td><td></td></tr><tr><td>ファルネゾール</td><td>シグマ・アルドリッチ</td><td>F203</td><td>95%</td></tr><tr><td>リナロール</td><td>シグマ・アルドリッチ</td><td>L2602</td><td>97%</td></tr><tr><td>ルリア・ベルタニ・ミディアム</td><td>ディフコ</td><td>244620</td><td></td></tr><tr><td>光学粘着フィルム</td><td>サーモフィッシャー</td><td>4311971</td><td>マイクロアンプ</td></tr><tr><td>レスベラトロール</td><td>シグマ・アルドリッチ</td><td>R5010</td><td>&ge;99%(HPLC)</td></tr><tr><td>Synergy HTX マルチモードリーダー</td><td>バイオテック</td><td></td><td></td></tr><tr><td>組織培養プレート 96 ウェル</td><td>カスヴィ</td><td>K12-096</td><td></td></tr></tbody></table>

chromobacterium violaceumにおけるビオラセインの定量と生理活性化合物による阻害

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

ここで示した結果には、私たちの研究グループ8によって以前に研究された3つの生理活性化合物、レスベラトロール、ファルネゾール、およびリナロールが使用されました。各化合物は、vioABCDE オペロン23 を介して QS システムによって調節される表現型である C. violaceum ATCC 12472 でビオラセイン産生を阻害する能力についてテストされました。
この結果は、ビオラセイン産生の定量化における当社のプロトコルの有効性と、 C. violaceum ATCC 12472 の生理活性化合物によるその阻害を検証します。細菌の増殖に影響を与えることなく、ファルネゾール、リナロール、およびレスベラトロールによるビオラセインレベルの有意な低下は、QSIを抗菌効果と区別する方法の能力を裏付けています24。以前の方法25、</sup...

Chromobacterium violaceumにおけるビオラセインの定量と生理活性化合物による阻害

Bacteria communicate through a system known as quorum sensing (QS), which allows them to coordinate their behavior in response to changes in cell density. ...

quantification-violacein-chromobacterium-violaceum-its-inhibition

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Biology

生物学

出典:Tay, S. B.ら。工学的クォーラムクエンチングラクトナーゼを用いた病原性バイオフィルムの抗病原性破壊。 J. Vis.

病原性細菌における酵素誘発バイオフィルム抑制の定量化

出典:Bucher, T.ら。B. subtilisバイオフィルムを小分子バイオフィルム阻害剤の特性評価モデルとして研究する方法。J. Vis. Exp.(2016年)このビデオでは、 Bacillus subtilis...

Bacillus subtilisにおけるペリクル形成に対する阻害剤の効果評価

ほとんどの細菌感染症はバイオフィルムを生成します。その環境のおかげで、バイオフィルム関連細菌はしばしば表現型的に薬剤耐性があります。したがって、バイオフィルム中の細菌を殺す新しい抗菌分子は、高い優先順位です。バイオフィルムの根絶に有効な抗菌化合物を迅速にスクリーニングするためのアッセイを確立します。...

バイオフィルム関連をターゲット<em&gt;黄色ブドウ球菌</em&gt;レサズリン基づく薬剤感受性アッセイを使用して

植物由来製品の抗真菌効果を定量化するために設計された修飾寒天ベースの方法について述べています。このプロトコルを通じて、抗真菌活性に対する揮発性および不揮発性の寄与の両方を評価することができる。さらに、真菌に対する有効性は、単一の実験セットアップの主要な発達段階で測定することができる。...

バイオコントロール製品の揮発性および不揮発性抗真菌活性の測定

この原稿は、単細胞蛍光顕微鏡を用いて、コカルチャーにおける細菌の競合を可視化し定量する方法を説明する。...

単細胞蛍光イメージングによる細菌間競争の定量化

アシネトバクターバイオフィルムの定量化、生存率評価、可視化戦略

チオフェネスルホンアミド化合物は、 ビブリオ クォーラムセンシングレギュレーターLuxR/HapRの強力で特異的な阻害剤であり、 in vivoでの活性を阻害し、病原性、運動性、およびバイオフィルムの遺伝子の転写を防ぎます。このプロトコールでは、これらの化合物がどのように合成され、 in silicoでモデル化され、in vivo...

ビブリオクォーラムセンシング阻害剤の合成とアッセイ

このプロトコルは、96ウェルプレートフォーマットとLEDパネル光源を組み合わせて、抗菌光線力学不活性化(aPDI)における潜在的な光増感剤の有効性を評価するための簡単で経済的な方法を説明しています。このアプローチにより、異なる濃度、化合物、細菌株など、複数の実験条件を同時に試験することができます。...

細菌の光力学的不活性化における光活性分子を評価するためのLEDベース In Vitro スクリーニング

本プロトコルは、細菌殺菌活性モデリングと組合せ相互作用アッセイを用いて、微生物制御を改善し全体的な殺菌剤使用量を減らす相乗効果を特定することを目的とした、植物化学物質が従来の殺菌剤の殺菌効果をどのように強化できるかを評価する体系的な枠組みを提供します。...

in vitro 植物由来分子が従来の殺生物剤に与える増強効果の評価

大 腸菌 細胞侵入の定量および可視化のためのアミカシン保護アッセイ

学ぶChromobacterium violaceumにおけるクォラムセンシング阻害とビオラセイン定量法。ファルネソールやリナロールなどの生理活性化合物を用いて、細菌コミュニケーションを標的とした新規抗病原性戦略の開発に貢献します。

Chromobacterium violaceumにおけるビオラセインの定量と生理活性化合物による阻害

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