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JoVE Journal
Medicine
インドシアニングリーン蛍光を用いた腹腔鏡下胆嚢摘出術:胆管鏡下結石摘出および一次管縫合
インドシアニングリーン蛍光を用いた腹腔鏡下胆嚢摘出術:胆管鏡下結石摘出および一次管縫合
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JoVE Journal Medicine
Laparoscopic Cholecystectomy with Indocyanine Green Fluorescence: Choledochoscopic Stone Extraction and Primary Duct Suture

インドシアニングリーン蛍光を用いた腹腔鏡下胆嚢摘出術:胆管鏡下結石摘出および一次管縫合

Full Text
616 Views
04:02 min
November 25, 2025

DOI: 10.3791/69432-v

Kaiwen Wu*1, Yujie Xu*2, Jiefeng Weng*2, Shian Wu1

1Department of General Surgery,Sihui People's Hospital, 2Hepatobiliary and Pancreatic Surgery Department,Guangzhou First People's Hospital

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Overview

This protocol presents an integrated technique for real-time biliary mapping using indocyanine green (ICG) fluorescence, facilitating minimally invasive stone clearance and secure primary duct repair.

Key Study Components

Area of Science

  • Minimally invasive surgery
  • Fluorescence imaging
  • Biliary tract procedures

Background

  • Common bile duct stones pose significant surgical challenges.
  • Traditional methods often require T-tube drainage.
  • Fluorescence cholangiography enhances visualization during surgery.
  • Direct visualization techniques improve outcomes in biliary surgery.

Purpose of Study

  • To optimize a minimally invasive protocol for stone removal.
  • To achieve primary duct closure without the need for T-tube drainage.
  • To utilize fluorescence imaging for precise surgical navigation.

Methods Used

  • Infraumbilical incision for trocar placement.
  • Use of multiple trocars for laparoscopic access.
  • Color fluorescence mode for imaging biliary structures.
  • Adjusting imaging gain for optimal visualization.

Main Results

  • Successful real-time biliary mapping achieved.
  • Minimally invasive stone clearance demonstrated.
  • Primary duct repair completed without T-tube drainage.
  • Enhanced visualization of biliary anatomy confirmed.

Conclusions

  • The integrated technique improves surgical outcomes.
  • Fluorescence imaging is effective for biliary procedures.
  • This protocol may reduce complications associated with traditional methods.

Frequently Asked Questions

What is the main advantage of using ICG fluorescence?
ICG fluorescence provides real-time visualization of biliary structures, enhancing surgical precision.
How does this protocol differ from traditional methods?
This protocol eliminates the need for T-tube drainage and focuses on minimally invasive techniques.
What are the key components of the surgical setup?
The setup includes multiple trocars and a laparoscope for guided surgery.
What is the significance of Calot's triangle in this procedure?
Calot's triangle is crucial for identifying biliary anatomy and ensuring safe dissection.
What imaging adjustments are necessary during the procedure?
Adjusting the gain to 50%-60% is recommended for optimal fluorescence imaging.
Can this technique be applied to other surgical procedures?
While primarily designed for biliary procedures, the principles may be adapted to other minimally invasive surgeries.

本プロトコルで説明されている統合技術は、インドシアニングリーン(ICG)蛍光によるリアルタイム胆道マッピング、直接可視下での低侵襲結石クリアランス、そして安全な一次管修復(6-0 PDS)を実現し、Tチューブ排出を除去します。

本研究は、低侵襲プロトコルを最適化し、一般的な胆管結石を安全に除去し、一次胆管閉鎖を達成することを目的としています。現在、現場では蛍光胆管造影と胆管鏡を組み合わせて精密な結石採取が行われています。まず、必要な下部切開を行い、最初の12ミリトロカーをオープンテクニックで挿入します。

腹腔鏡の指導のもと、さらに4本のトロカーを挿入します。1本は上腹部、1本は右鎖骨中線の肋下線、右前腋下線に1本、左の副正中点(歯骨と臍骨の中間)に1本ずつ配置します。カメラモードとボタンを一度押すと、カラー蛍光モードに切り替わります。ゲインを50%から60%に調整してください。カロ氏三角形の構造を観察し、嚢胞管、総肝管、総胆管の蛍光イメージングを確認してください。

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