Name: マウスの筋肉および末端シュワン細胞におけるミトコンドリア形態の 現場 定量方法 (動画) | JoVE Journal
Uploaded: 2026-04-10T13:00:00.000+00:00
Duration: 8 min 13 s
Description: 学ぶマウスの骨格筋と末端シュワン細胞におけるミトコンドリア形態の現場定量法。膜電位感受性蛍光色素を用いたin vivo標識と共焦点顕微鏡による三次元イメージングで、神経筋接合部のミトコンドリアネットワークを詳細に解析できます。

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</ol>

<table><tbody><tr><td>3-in-1 Whole Animal System</td><td>Aurora Scientific, Ontario, Canada</td><td>1300A</td><td>筋機能システムと分析ソフトウェア</td></tr><tr><td>AIVIA&nbsp;</td><td>Leica Microsystems, Bellevue, WA, USA</td><td>15</td><td>AI駆動型画像解析ソフトウェア</td></tr><tr><td>anti-laminin&nbsp;</td><td>MilliporeSigma</td><td>L9393-.2mL</td><td>筋線維の境界ラベル</td></tr><tr><td>B6; D2-Tg(S100-EGFP)1Wjt/J Mouse Strain</td><td>Jackson Labs, Bar Harbor, ME</td><td>JAX:005621</td><td>S100&beta;</td></tr><tr><td>Cryostar NX50 Cryostat</td><td>Epredia, Kalamazoo, MI, USA</td><td>https://www.epredia.com/products/histology-instruments/cryotomy/cryostar-nx50</td><td>凍結切片装置</td></tr><tr><td>D2.B10-DMDD2.mdx/J Mouse Strain</td><td>Jackson Labs, Bar Harbor, ME</td><td>JAX:13141</td><td>D2.mdx</td></tr><tr><td>DBA/2J Mouse Strain</td><td>Jackson Labs, Bar Harbor, ME</td><td>JAX:00671</td><td>WT</td></tr><tr><td>Dimethyl Sulfoxide (DMSO)</td><td>Fisher Scientific; Hampton, NJ, USA</td><td>BP231-100</td><td>ミトコンドリア染色再構成試薬</td></tr><tr><td>Hoechst 33342</td><td>Thermo Fisher Scientific</td><td>H1399</td><td>細胞透過性核酸染色</td></tr><tr><td>ImageJ/Fiji&nbsp;</td><td>National Institutes of Health, Bethesda, MD, USA</td><td>SCR_002285</td><td>2D解析ソフトウェア</td></tr><tr><td>Invitrogen ProLong Gold antifade reagent with DAPI&nbsp;</td><td>Fisher Scientific, Hampton, NJ, USA</td><td>P36941</td><td>実装媒体</td></tr><tr><td>KimWipe</td><td>Kimberly-Clark, Irving, TX, USA</td><td>06-666A</td><td>実験室用ワイプ</td></tr><tr><td>Leica LAS_X software&nbsp;</td><td>Leica Biosystems, Wetzlar, Germany</td><td>SCR_013673</td><td>共焦点ソフトウェア</td></tr><tr><td>MitoView Fix 640</td><td>Biotium</td><td>70075-50ug</td><td>遠赤色蛍光ミトコンドリア染色</td></tr><tr><td>NIS-Elements Advanced Research software&nbsp;</td><td>Nikon, Melville, NY, USA</td><td>SCR_014329</td><td>断面積解析ソフトウェア</td></tr><tr><td>Permount Mounting Medium&nbsp;</td><td>Fisher Scientific, Waltham, MA 02454, USA</td><td>#SP15-100</td><td>一般実装媒体</td></tr><tr><td>Prism 9 software&nbsp;</td><td>GraphPad Software, La Jolla, CA, USA</td><td>RRID: SCR_002798</td><td>統計解析ソフトウェア</td></tr><tr><td>Rhodamine (TRITC)</td><td>Fisher Scientific; Hampton, NJ, USA</td><td>AP132RMI</td><td>二次抗体</td></tr><tr><td>Stellaris 5 White Light Confocal Microscope and corresponding deconvolution software</td><td>Leica Microsystems, Wetzlar, Germany</td><td>158301313:158201310:158004752: 158301312:158301314:158401100: 158209011:158301130:158204510: 158301120:158401140:158204511: 15500332:15506428:15506517:155 13859:15521522:15525226:155252 32:15525314:15555009:15555017: 15555046:27100022:155933660: 155933666:155933668:158000640 :158002407:158003150:158004141 :158004201:158004421:158004423 :158200681:158202140:158203114: 158203200:158203220:158204201: 158204510:158204709:158301200: 9I_LL_STELLARIS_C:9T-CLSM-APP _CLASSC:9WE_LAS_405:9WE_ LAS_WLL:9WE_LL_STELLARIS_C</td><td>共焦点顕微鏡</td></tr><tr><td>SZ61</td><td>Olympus, Breinigsville, PA, USA</td><td>sz61</td><td>立体顕微鏡</td></tr><tr><td>Tissue-Plus O.C.T. Compound&nbsp;</td><td>Scigen, Fischer Scientific, Hampton, NJ, USA</td><td>23-730-571</td><td>最適切断温度化合物</td></tr><tr><td>Visikol I</td><td>Sigma-Aldrich, St. Louis, MO, USA</td><td>H1-30</td><td>組織クリアリング溶液 1</td></tr><tr><td>Visikol II</td><td>Sigma-Aldrich, St. Louis, MO, USA</td><td>H2-30</td><td>組織クリアリング溶液 2</td></tr><tr><td>&alpha;-bungarotoxin 552</td><td>Biotrend, Koln, Germany</td><td>00014</td><td>アセチルコリン受容体染料</td></tr></tbody></table>

マウスの筋肉および末端シュワン細胞におけるミトコンドリア形態の 現場 定量方法

Methods for In situ Quantification of Mitochondrial Morphology in Muscle and Terminal Schwann Cells of Mice

Mitochondrial network morphology is widely used as an indicator of cellular health; however, quantifying mitochondrial architecture within intact tissues ...

methods-for-situ-quantification-mitochondrial-morphology-muscle

Research

JoVE Journal

Biology

生物学

このような神経筋接合部（NMJs）で端末シュワン細胞（SCS）と密集した組織内の個々の細胞を可視化に挑戦している。...

シーケンシャル神経筋接合部におけるシングルシュワン細胞の輪郭を描くのにフォトブリーチング

骨格筋は歩行のために不可欠であり、団体「メインタンパク質店です。 C.エレガンス内の筋肉の健康の測定について説明する。筋肉の構造と機能の将来の変更は、ローカライズされたGFPおよびカチオン染料を使用して評価される。...

筋肉の細胞内コンパートメントを評価するための方法 C。エレガンス

このプロトコルは、神経固有のミトコンドリアを定量化を視覚化して三次元 (3 D) 画像処理・解析技術を使用します。テクニックは別の蛍光信号からデータのサブセットを分離する 1...

三次元画像と人間の表皮内神経線維内のミトコンドリアの解析

哺乳類の脳組織からのミトコンドリアの可視化と分析は困難な作業です。ここでは、シリアルブロック面走査型電子顕微鏡(SBFSEM)による3次元(3D)再構成解析を使用して、この重要なエネルギー生成オルガネラの形態学的および体積的解析に関する洞察を得る方法について説明します。...

シリアルブロック・フェイス走査型電子顕微鏡を用いて脳ミトコンドリアの解析

我々は、アスコルビン酸ペルオキシダーゼ2およびワサビペキシダーゼ染色を含む相関光および体積電子顕微鏡を用いて遺伝子改変後の全細胞におけるミトコンドリアおよび小和性網膜の分布を研究するためのプロトコルを提示し、同じセクション内の標的遺伝子の有無にかかわらず細胞のシリアル断面、および電子顕微鏡によるシリアルイメージング。...

相関光と体積電子顕微鏡によるミトコンドリアと小体網膜イメージング

本研究では、自由に利用可能な画像処理ツールを用いて、C.エレガンスにおけるシナプスサイズと局在化、筋肉形態、ミトコンドリア形状の定量的測定を概説する。このアプローチにより、C.エレガンスにおける将来の研究は、遺伝的変異の結果としての組織および小器官構造変化の程度を定量的に比較することを可能にする。...

カエモルハブ炎エレガンスにおける細胞構造と器官形態学の研究に関する定量的アプローチ

神経筋接合成分を正確に検出する能力は、病理学的または発達的プロセスのために、そのアーキテクチャの変更を評価する上で非常に重要です。ここでは、定量的測定を行うために使用できる全マウント神経筋接合部の高品質画像を得るための簡単な方法の完全な説明を提示する。...

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このプロトコルは、SMAおよびColQ関連のCMSのマウスモデルの病理学的変化を定量化するために使用される共焦点顕微鏡とSTED顕微鏡の組み合わせによる神経筋接合部の形態測定分析の方法を説明しています。...

共焦点顕微鏡と超解像顕微鏡の組み合わせによるマウスの神経筋接合部のキャラクタリゼーション

このプロトコルは、老化中の C.エレガンスの...

老化中の C.エレガンス におけるミトコンドリア形態のイメージングと定量化

以下のプロトコールは、膵臓の仮想スライスイメージングのための解剖、および全体の膵臓内のすべてのGFPタグ付きβ-細胞のその後の定量化のプロセスを概説します。...

マウスにおける膵β細胞の質量のその場定量に

学ぶマウスの骨格筋と末端シュワン細胞におけるミトコンドリア形態の現場定量法。膜電位感受性蛍光色素を用いたin vivo標識と共焦点顕微鏡による三次元イメージングで、神経筋接合部のミトコンドリアネットワークを詳細に解析できます。

マウスの筋肉および末端シュワン細胞におけるミトコンドリア形態の現場定量方法

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マウスの筋肉および末端シュワン細胞におけるミトコンドリア形態の 現場 定量方法

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マウスの筋肉および末端シュワン細胞におけるミトコンドリア形態の現場定量方法