Name: 自己組織化ヒト心型心肌における低酸素再酸素損傷モデル (動画) | JoVE Journal
Uploaded: 2026-03-17T12:35:00.000+00:00
Duration: 10 min 41 s
Description: 学ぼう、iPSC由来自己組織化心型オルガノイドを用いた低酸素再酸素損傷モデルの構築法。虚血再灌流損傷を模倣し、組織学的・機能解析で心臓傷害応答と再生医療の評価を可能にする。

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</ol>

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自己組織化ヒト心型心肌における低酸素再酸素損傷モデル

A Hypoxia-Reoxygenation Injury Model in Self-Assembling Human Cardioids

Without rapid revascularization, ischemic heart disease leads to irreversible loss of cardiomyocytes, scar formation, and decreased cardiac function. The ...

a-hypoxia-reoxygenation-injury-model-self-assembling-human

Research

JoVE Journal

Medicine

医学

この原稿は、表面マーカー発現と細胞選別を使用して、定義された人工心臓組織の作成について説明しています。次に、定義された組織をマルチティッシュバイオリアクターで使用して、心臓細胞療法のメカニズムを調査し、機能的でありながら制御されたヒト心臓のモデルシステムを提供できます。...

心臓細胞療法のメカニズムを研究するために定義されたヒューマン・エンジニア心臓組織の構築

ここでは、輸血やラットの変力作用エージェントなしの単純復旧心肺モデルを説明するプロトコルを提案します。このモデルでは、長期の研究人工心肺の複数臓器の後遺症をことができます。...

輸血やラットの変力作用エージェントなし回復心肺モデル

ヒト人工多能性幹細胞由来の心筋細胞を用いた虚血性心疾患のモデルを、虚血による組織損傷の定量的評価方法と共に提示する。このモデルは、薬物スクリーニングおよび虚血性心疾患に関するさらなる研究のための有用なプラットフォームを提供することができる。...

hiPSC由来心筋細胞を用いた虚血性心疾患のモデルと心筋細胞収縮の映像的比較

心筋保護に対する低体温の異なる程度の明確な影響は十分に評価されていない。本研究の目的は、ヒト心筋細胞系モデルにおける異なる低体温症治療に続く細胞死のレベルを定量化し、将来の詳細な分子研究の基礎を築くことであった。...

ヒト心臓筋細胞モデルにおける低体温症前処理に対する心筋保護のインビトロ評価

このプロトコルの目的は、生体整血、コラーゲンベースのヒドロゲル、心臓組織工学、薬物スクリーニング、および疾患モデリングにおける応用のために、心臓線維芽細胞(CF)と共培養された幹細胞由来心筋細胞で構成される、高度に整列したヒト心臓組織の三次元(3D)微小流体モデルの開発を説明し、実証することである。...

マイクロ流体プラットフォーム内での3D組織化ヒト心臓組織の開発

空間距離は、別々の内皮細胞層と心筋細胞層の共培養モデルにおける低酸素/再酸素化傷害を評価する際の重要なパラメータであり、心筋細胞保護における内皮細胞の役割を試験するための好ましい in vitro...

インビトロで心臓虚血/再灌流を模倣する細胞共培養モデルの開発

ここでは、自己組織化によってヒト多能性幹細胞を用いて、発達的に関連するヒト心臓オルガノイド(hH)を効率的に作成するためのプロトコルについて述べた。このプロトコルは、発達的手がかりの逐次活性化に依存し、非常に複雑で機能的に関連するヒト心臓組織を生成する。...

多能性幹細胞由来の自己組織化ヒト心臓オルガノイドの生成

幹細胞由来の心筋細胞を用いてバイオエンジニアリングされた3次元心臓組織は、健康なヒト心筋と病気のヒト心筋を in vitro...

人工心臓組織のデータ取得とモデルスループットを向上させるバイオリアクターの設計

ここでは、代謝性の高い近位管上皮細胞における低酸素/再酸素化損傷をモデル化する in vitro...

近位管上皮細胞における低酸素症/再酸素化損傷のモデル化

重度の脊髄損傷の挫傷モデルが記載されている。詳細、術前手術と術後の手順は一貫したモデルを得るために説明されています。...

ラットにおける重度の脊髄損傷挫傷モデル

学ぼう、iPSC由来自己組織化心型オルガノイドを用いた低酸素再酸素損傷モデルの構築法。虚血再灌流損傷を模倣し、組織学的・機能解析で心臓傷害応答と再生医療の評価を可能にする。

自己組織化ヒト心型心肌における低酸素再酸素損傷モデル

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