Name: 妊娠前の炎症が神経発達に与える評価のための早期妊娠母体免疫活性化マウスモデルの生成 (動画) | JoVE Journal
Uploaded: 2026-03-24T13:05:00.000+00:00
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Description: 学ぶ早期妊娠母体免疫活性化マウスモデルの標準化プロトコル。poly(I:C)を用いた神経発達障害研究手法で、サイトカイン定量と体重推移による品質管理で再現性を向上させる。

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</ol>

<table><tbody><tr><td>動物体重計</td><td>Kent Scientific</td><td>SCL-4000</td><td>マウスの体重測定用</td></tr><tr><td>BD&nbsp;Lo-Dose U-100 インスリン注射器 - 28G</td><td>Fisher Scientific</td><td>14-826-79</td><td>PIC、腹腔内注射用</td></tr><tr><td>C57BL/6J メスマウス</td><td>The Jackson Laboratory</td><td>000664</td><td>10-12週齢、処女メス</td></tr><tr><td>C57BL/6J オスマウス&nbsp;</td><td>The Jackson Laboratory</td><td>000664</td><td>繁殖用オス</td></tr><tr><td>C57BL/6NCrl メスマウス</td><td>Charles River Laboratories</td><td>027</td><td>10-12週齢、処女メス</td></tr><tr><td>遠心分離機（24ポット標準型）</td><td>Eppendorf</td><td>5425 R</td><td>血液を遠心分離して血清を採取</td></tr><tr><td>データ文書化プラットフォーム&nbsp;</td><td>Airtable、Microsoft Excelなど</td><td></td><td>重量、注射時間などの重要なデータの記録（表1）</td></tr><tr><td>デジタルドライバス - 1ブロック</td><td>Medicus Health</td><td>5277M1</td><td>注射前のPIC加熱用</td></tr><tr><td>Eppendorfチューブ - 1.5 mL</td><td>Eppendorf</td><td>30123611</td><td>PICをストック濃度および作業濃度に希釈する際の使用</td></tr><tr><td>Fisherbrand ガーゼスポンジ</td><td>Fisher Scientific</td><td>MSD-1400250</td><td>採血後の尾の止血用</td></tr><tr><td>Fisherbrand マールプローブ 6インチ</td><td>Fisher Scientific</td><td>13-820-026</td><td>チェックを塞ぐ用途</td></tr><tr><td>HydroGel</td><td>Clear H20</td><td>70-01-5022</td><td>注射後の補給</td></tr><tr><td>Mouse IL-6 uncoated ELISA kit</td><td>Thermo Fisher Scientific</td><td>88-7064-88</td><td>母体血清中のIL-6レベルの定量</td></tr><tr><td>Nalgene ポリプロピレン Griffin ロウフォームプラスチックビーカー</td><td>Thermo Fisher Scientific</td><td>1201-1000PK</td><td>体重測定時のマウスの保持用</td></tr><tr><td>Poly(I:C) (LMW)</td><td>Invivogen</td><td>tlrl-picw</td><td>MIA注射用PIC (LMW)</td></tr><tr><td>ポリイノシン酸-ポリシチジン酸ナトリウム塩</td><td>Sigma</td><td>P9582</td><td>MIA注射用PIC (Sigma)</td></tr><tr><td>メスカルブレード、#22、10パック</td><td>Home Science Tools</td><td>DE-SC22BLD</td><td>採血用の尾の切断用途</td></tr><tr><td>塩化ナトリウム0.9%（生理食塩水）、USP、無菌グレード、Cytiva Hyclone</td><td>Cytiva</td><td>Z1376</td><td>対照マウスの生成用注射</td></tr><tr><td>尾切断プラットフォーム</td><td>Braintree Scientific, Inc.</td><td>IL-200</td><td>尾の採血時のマウスの適切な拘束</td></tr><tr><td>Vortex-Genie 2</td><td>Scientific Industries, Inc.</td><td>SI-0236</td><td>希釈後のPICの渦流混合</td></tr></tbody></table>

妊娠前の炎症が神経発達に与える評価のための早期妊娠母体免疫活性化マウスモデルの生成

Generation of the Early-Gestational Maternal Immune Activation Mouse Model to Assess Prenatal Inflammation on Neurodevelopment

Neurodevelopmental disorders (NDDs) arise from complex interactions between genetic and environmental risk factors, including prenatal immune activation. ...

generation-early-gestational-maternal-immune-activation-mouse-model

Research

JoVE Journal

Neuroscience

神経科学

未熟児の脳症は、早産による損傷に関連する中枢神経系の異常を包含します。...

ラットにおける羊膜内リポ多糖で出生前低酸素性虚血を使用した未熟児のモデル化脳症

母体の免疫活性化（MIA）は、自閉症と統合失調症の環境リスク因子のモデルです。この記事の目的は、このモデルの再現性を高めるために、妊娠中のマウスでMIAを誘導する方法の段階的な手順を提供することです。...

ウイルスミミックポリ（I：C）で、妊娠中期の段階でのマウスにおける母性免疫活性化の誘導

生きた生物の合併症を伴わない胎児の母体炎症(FEMI)をシミュレートする絨毛膜炎のモデルを開発し、子孫の腸管の発達に及ぼすFEMIの影響を調べた。これは、絨毛膜炎後の腸の損傷の発症のための機械化原因の研究を可能にする。...

新生児腸管の発達に及ぼす急性絨毛膜炎の影響を研究する母体炎症への胎児暴露のマウスモデル

慢性心理社会的ストレス(CGS)パラダイムは、母親と乳児の精神疾患をモデル化するために、マウスで妊娠中に臨床的に関連するストレータを採用しています。ここでは、このモデルを検証するためにCGSパラダイムとダウンストリーム評価を適用するステップバイステップの手順を提供します。...

母親と乳児の精神疾患に対する翻訳的に関連するパラダイムとしての妊娠における心理社会的ストレスのマウスモデルの使用

母体感染は神経発達障害の危険因子です。母体免疫活性化(MIA)のマウスモデルは、感染が脳の発達と機能に与える影響を解明する可能性があります。ここでは、MIAにさらされた確実に回復力があり感受性のある子孫を産出するための一般的なガイドラインと手順が提供されています。...

ポリ(I:C)を用いた妊娠中期の母体免疫活性化の再現モデルの生成による子孫の感受性と回復力の研究

マウスにおける上行性膣感染症、早産、および新生児罹患率のモデル化

片頭痛と慢性片頭痛への変換が改善された治療の選択肢の必要性の巨大なヘルスケアの負担です。我々は、モデル化、神経免疫シグナル伝達が片頭痛に対する感受性を調節する方法を定義するために模索...

拡散うつ病を使用してエピソードと慢性片頭痛の神経免疫シグナルのモデリングインビトロ</em

中枢神経系の再生および修復を調査するために、ショウジョウバエの幼虫腹神経索を用いた傷害のパラダイムが記載されている。意図的に開発されたソフトウェアと遺伝との定量的な分析と組み合わせタイムラプスおよび固定標本でレーザー走査共焦点顕微鏡法、続いて刺すことは中枢神経系の再生および修復の分子メカニズムを解明するために使用されます。...

中枢神経系の修復を調査するために傷害パラダイム<em&gt;ショウジョウバエ</em

後根神経節（DRG）、末梢神経系の感覚ニューロンを含む構造である。解離した場合、それらは損傷部位でのインビボ環境を模倣し、in vitroでの神経再生及び髄鞘形成を研究するための貴重なモデルを提供し、SC-ような脂肪由来幹細胞（ASC）と共培養することができる。...

根神経節ニューロンと差別化された脂肪由来幹細胞を背：<em&gt;インビトロ</em末梢神経再生を研究するために&gt;共培養モデル

ここでは、アミロイドベータの脳定位固定剤を直接注入するためのプロトコルを紹介します。この方法論は、アミロイドベータが脳ニューロンに及ぼす短期的な影響に対処するための代替のin...

マウス海馬にオリゴマーアミロイドベータの定位注入

学ぶ早期妊娠母体免疫活性化マウスモデルの標準化プロトコル。poly(I:C)を用いた神経発達障害研究手法で、サイトカイン定量と体重推移による品質管理で再現性を向上させる。

妊娠前の炎症が神経発達に与える評価のための早期妊娠母体免疫活性化マウスモデルの生成

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