液滴界面二重層を用いた脂質-タンパク質膜構造-機能の特徴解析

461 回視聴

10:27 分

2026年6月12日

461 回視聴

Peter T. Podar¹^,² , Ariana Adkisson-Washington²^,³ , Olivia Ziemer²^,⁴ , Victoria Sullivan²^,⁵ , Christian Milem²^,⁶ , Janine Hagar-Montoya²^,⁷ , Shriya Khule²^,⁸ , Zachary Griffith²^,⁹ , Khalid Tantawi²^,¹⁰ , Xiuhong Zhai²^,¹¹ , Dima Bolmatov¹² , John Katsaras²^,¹¹ , Charles Patrick Collier¹³

¹Carle Illinois College of Medicine, University of Illinois at Urbana-Champaign, ²Shull Wollan Center, University of Tennessee, ³Department of Chemistry, Louisiana State University, ⁴Department of Physics and Astronomy, University of Tennessee, ⁵Department of Chemical and Biomolecular Engineering, University of Tennessee, ⁶Department of Chemical and Environmental Engineering, University of Cincinnati, ⁷Department of Metallurgical, Materials, and Biomedical Engineering, University of Texas at El Paso, ⁸Farragut High School, ⁹Department of Chemical and Biological Engineering, University of Alabama, ¹⁰Department of Engineering Management and Technology, University of Tennessee, ¹¹Labs and Soft Matter Group, Oak Ridge National Laboratory, ¹²Department of Physics and Astronomy, Texas Tech University, ¹³Center for Nanophase Materials Sciences, Oak Ridge National Laboratory

グラミキシジンAドープされた液滴界面二重層を組み立て、電気刺激し、解析する実験プロトコルを提示します。脂質-タンパク質の構造-機能関係は、膜面積、イオンフラックス、単一チャネル伝導の変化を測定し、これらの応答を電気シナプスに着想を得た膜モデルにおける可塑性様のイオン伝導変化と関連付けることで定量化されます。