潜在的な抗結核候補の同定:哺乳類細胞における合成、MIC決定、細胞傷害評価のステップバイステップガイド
428 回視聴
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10:24 分
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2026年5月22日
DOI :10.3791/70693-v
2026年5月22日
•428 回視聴
Alexia de Matos Czeczot*1,2 , Ariel Moura Maia*1 , Camili Zanella Zotti*1 , Guilherme Arraché Gonçalves*1 , Josiane Delgado da Paz*1 , Marcia Alberton Perelló*1 , Nicholas Kraether Roth*1,2 , Luiz Augusto Basso1,2,3 , Pablo Machado1,2,3 , Cristiano Valim Bizarro1,2
1Center for Molecular and Functional Biology Research, National Institute of Science and Technology in Tuberculosis, Pontifical Catholic University of Rio Grande do Sul, 2Graduate Program in Cellular and Molecular Biology, School of Health and Life Sciences, Pontifical Catholic University of Rio Grande do Sul, 3Graduate Program in Medicine and Health Sciences, School of Medicine, Pontifical Catholic University of Rio Grande do Sul
* These authors contributed equally
ここでは、4-アミノキノリン化合物の化学合成、結核菌に対する最小阻害濃度の決定、哺乳類細胞株における細胞毒性の評価を含む表現型スクリーニングを通じて潜在的な抗結核薬候補を特定するプロトコルを提示します。
Chapters in this video
0:00
Introduction
0:22
Synthesis of 6-Bromo-2-methyl-N-(4-(piperidin-1-yl)phenyl)quinolin-4-amine
1:37
Characterization of Compound 6 Using HPLC
2:36
Nuclear Magnetic Resonance (1H, 13C) Analysis of Compound 6
3:22
Purification of Compound 6 by Column Chromatography
3:49
Minimal Inhibitory Concentration Determination Using Resazurin Assay
5:37
Cytotoxicity Assessment by MTT Assay
6:49
Neutral Red Uptake Assay for Cytotoxicity Assessment
8:09
Results
9:52
Conclusion
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